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Colorimetric detection method for nitrite in water

A technology of nitrite and detection method, applied in the field of analytical chemistry, can solve problems such as secondary pollution of the environment, and achieve the effects of simple operation, simple and cheap instruments and equipment, and simplified detection process

Active Publication Date: 2018-11-02
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the Griess method involves two reagents, 4-aminobenzenesulfonamide and N-1-naphthylethylenediamine, and requires a two-step reaction, requiring an acidic environment
In addition, N-1-naphthylethylenediamine reagent is a carcinogen and will cause secondary pollution to the environment

Method used

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  • Colorimetric detection method for nitrite in water
  • Colorimetric detection method for nitrite in water
  • Colorimetric detection method for nitrite in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Utilize the aqueous solution of 2mM 2-amino-6-chlorobenzoic acid to detect the concentration of nitrite ion

[0031] The selected reagent is 2-amino-6-chlorobenzoic acid, that is, when the R substituent in the compound of general formula I is chlorine, the molecular weight is 171.

[0032] Preparation of 2-amino-6-chlorobenzoic acid aqueous solution: Weigh 171mg of 2-amino-6-chlorobenzoic acid, dissolve it in double distilled water or deionized water, and make the volume to 500mL, with a final concentration of 2mM.

[0033] Prepare NaNO 2 Standard solution: Weigh 172.5 mg of sodium nitrite (molecular weight: 69), dissolve it in double distilled water or deionized water, and then dilute it to 500 mL with double distilled water or deionized water step by step to 13 different concentration gradients of NaNO2 The standard solutions are 2, 4, 6, 8, 10, 15, 20, 25, 30, 35, 40, 45 and 50 (in mM), each standard solution is 50mL, and the final concentration concen...

Embodiment 2

[0036] Embodiment 2: Utilize the aqueous solution of 0.5mM 2-amino-6-chlorobenzoic acid to detect the concentration of nitrite ion

[0037] The selected reagent is 2-amino-6-chlorobenzoic acid, that is, when the R substituent in the compound of general formula I is chlorine, the molecular weight is 171.

[0038] Preparation of 2-amino-6-chlorobenzoic acid aqueous solution: Weigh 43mg of 2-amino-6-chlorobenzoic acid, dissolve it in double distilled water or deionized water, and adjust the volume to 500mL with a final concentration of 0.5mM.

[0039] Prepare NaNO 2 Standard solution: Weigh 172.5 mg of sodium nitrite (molecular weight: 69), dissolve it in double distilled water or deionized water, and then dilute to 500 mL with double distilled water or deionized water step by step to 12 different concentration gradients of NaNO2 The standard solutions are 0, 1, 2, 3, 4, 6, 8, 10, 15, 20, 25 and 30 (in mM), each standard solution is 50mL, and the final concentration gradient can...

Embodiment 3

[0042] Embodiment 3: Utilize the aqueous acetic acid solution of 2mM 2-amino-6-chlorobenzoic acid to detect the concentration of nitrite ion

[0043] The selected reagent is 2-amino-6-chlorobenzoic acid, that is, when the R substituent in the compound of general formula I is chlorine, the molecular weight is 171.

[0044] For the preparation of 2-amino-6-chlorobenzoic acid acetic acid aqueous solution, the concentration of 2-amino-6-chlorobenzoic acid is generally controlled at 0-30mM, the concentration of acetic acid is generally 0.1-1M, and the pH value is 3.0-4.0. Accurately measure 40mL of glacial acetic acid, dilute to 1000mL with double distilled water or deionized water, the final concentration of acetic acid aqueous solution is 0.7M; weigh 171mg of 2-amino-6-chlorobenzoic acid, dissolve it with 0.7M Make up to 500mL to obtain 2-amino-6-chlorobenzoic acid acetic acid aqueous solution.

[0045] Prepare NaNO 2 Standard solution: Weigh 172.5 mg of sodium nitrite (molecul...

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Abstract

The scheme discloses a colorimetric detection method for nitrite in water in the technical field of analytical chemistry. The colorimetric detection method comprises the following steps: using compound solution with the formula I (which is shown in the description) as a detection reagent, weighing 172.5 mg of sodium nitrite, setting the volume to 500 mL after dissolving by using double-distilled water, and then using the double-distilled water to dilute to be multiple NaNO2 standard solutions with different concentration gradients, wherein each standard solution is 50 mL; taking the detectionreagent and the NaNO2 solution with the standard concentration, and mixing according to the proportion of 1:1; using an ultraviolet lamp to irradiate the mixed solution for 100 minutes; utilizing a spectrophotometer to measure the absorption value of the mixed slution at 400-550 nm; establishing a standard curve between nitrite ions and the absorbing value according to the measured absorption value and the known nitrite-ion concentration; carrying out determination on the detected sample; in the determination process, controlling the detection value of the sample to be in the detection range of the standard curve; calculating the molar concentration of the nitrite ions in the sample to be detected according to the linear equation of the standard curve and the dilution ratio of the sample.The colorimetric detection method disclosed by the scheme is simple in operation and low in detection cost.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a colorimetric detection method for nitrite in water quality. Background technique [0002] Nitrite is an inorganic salt of white crystalline powder, easily soluble in water, and its aqueous solution is colorless and odorless. Nitrite is widely used in all walks of life. In industry, nitrite is often used in color development and pickling preparations of meat products to improve flavor, color, and quality. In agriculture, the use of chemical fertilizers is not long. However, with the excessive use of nitrogen fertilizers, the rate of nitrogen absorption by crops is much greater than the rate of reduction of nitrate nitrogen in crops, resulting in the accumulation of nitrite; and the use of a large number of phosphorus detergents in daily life makes domestic sewage often contain excessive N, P, etc. Nutrients will cause eutrophication of water bodies. [0003] Nitri...

Claims

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Application Information

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IPC IPC(8): G01N31/22
CPCG01N31/227
Inventor 万南微陈永正王慧慧
Owner ZUNYI MEDICAL UNIVERSITY
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