Tissue culture seedling raising method of drosera burmanii

A technology of tissue culture and jindiluo, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of inability to adapt to market demand, low survival rate, low yield, etc., and achieve good disinfection and sterilization effects, The effect of high induction rate and high differentiation rate

Inactive Publication Date: 2018-11-06
广西中农富玉国际农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most of the existing Jindiluo seedling cultivation methods are direct planting of seeds, with low yield and low s

Method used

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  • Tissue culture seedling raising method of drosera burmanii
  • Tissue culture seedling raising method of drosera burmanii

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0022] Example 1

[0023] A method for tissue culture and seedling cultivation of Jindiluo includes the following steps:

[0024] S1: Disinfect the tissue culture operation room and culture room with Synergine to ensure that the working area of ​​the workbench is in a sterile environment;

[0025] S2: Select the pedicels of fresh, uncracked plants of Jindiluo, wash them with laundry detergent, rinse for 15-30 minutes, soak the pedicels with 70% sodium hypochlorite solution on a sterile ultra-clean workbench for 3 minutes, and then use Rinse it with sterile water for 5 times and place it in a sterilized petri dish, place it directly on the ultra-clean workbench, and cut into stem segments with a length of 1 cm as explants for later use;

[0026] S3: Preparation of medium: The medium is 1 / 4MS medium formula + 28g / L sugar + 0.3mg / L 6-BA+0.15mg / L NAA hormone +0.04Ac+5g / L agar, and the above substances are packed Put it into the culture flask to adjust the pH to 5.8 and then seal it, then...

Example Embodiment

[0030] Example 2

[0031] A method for tissue culture and seedling cultivation of Jindiluo includes the following steps:

[0032] S1: Disinfect the tissue culture operation room and culture room with Synergine to ensure that the working area of ​​the workbench is in a sterile environment;

[0033] S2: Select the pedicels of fresh, uncracked plants of Jindiluo, wash them with laundry detergent, rinse for 30 minutes, soak the pedicels with a 75% mass concentration sodium hypochlorite solution on a sterile ultra-clean workbench for 5 minutes, and then use aseptic Rinse it with water for 6 times and place it in a sterilized petri dish, place it directly on the ultra-clean workbench, and cut into stem segments with a length of 1 cm as explants for later use;

[0034] S3: Preparation of medium: The medium is 1 / 4MS medium formula + 32g / L white sugar + 0.6mg / L 6-BA+0.25mg / L NAA hormone +0.055% Ac+7g / L agar, and the above substances Put it into a culture flask to adjust the pH to 6.5 and then...

Example Embodiment

[0038] Example 3

[0039] A method for tissue culture and seedling cultivation of Jindiluo includes the following steps:

[0040] S1: Disinfect the tissue culture operation room and culture room with Synergine to ensure that the working area of ​​the workbench is in a sterile environment;

[0041] S2: Select the pedicels of fresh, uncracked plants of Jindiluo, wash them with laundry detergent, rinse for 18 minutes, soak the pedicels with 72% sodium hypochlorite solution on a sterile ultra-clean workbench for 4 minutes, and then use aseptic Rinse it with water for 6 times and place it in a sterilized petri dish, place it directly on the ultra-clean workbench, and cut into stem segments with a length of 1 cm as explants for later use;

[0042] S3: Making culture medium: The culture medium is 1 / 4MS medium+30g / L sugar+0.5mg / L 6-BA+0.2mg / LNAA hormone+0.05%Ac+6g / L agar, and the above substances are loaded Adjust the pH value in the culture flask to 6.2 and then seal it, then steam steriliz...

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Abstract

The invention provides a tissue culture seedling raising method of drosera burmanii and belongs to the field of planting of drosera burmanii. The method comprises the steps as follows: firstly, pedicels of fresh and non-cracked plants are selected and cleaned, disinfection is performed with a sodium hypochlorite solution, the disinfectant on the surfaces of the pedicels is washed away by flowing water, and sterile explants are obtained; the explants are illuminated in a culture medium at constant temperature and humidity, seedlings are obtained, and the seedlings are firstly placed in a greenhouse for acclimation for a period and then transferred in a cultivation matrix. By the aid of the tissue culture seedling raising method of drosera burmanii, the drosera burmanii can propagate rapidly, the survival rate of the drosera burmanii is greatly increased, and the method is simple to operate, low in cost and applicable to mass systematic planting and can increase yield and income of farmers.

Description

【Technical field】 [0001] The invention relates to the technical field of jindiluo planting, in particular to a method for raising jindiluo seedlings through tissue culture. 【Background technique】 [0002] Jindiluo is an insectivorous plant that grows on grassland or wet rock surfaces and sandy soil; it belongs to herbaceous plants with short stems and no bulbs. The leaves are covered with glandular hairs on the edge. When insects fall in, the glandular hairs will surround the insects, and the sticky glands will stick the insects. The secreted liquid can decompose nutrients such as insect body proteins, and then absorbed by the leaves; Ye Te to form an insect trap. Jindiluo is an endangered wild ornamental and medicinal plant. Wild species are becoming rarer and rarer. It can be used as a small potted plant for home gardening. It can not only prey on insects but also produce beautiful flowers. Jindiluo also has the effect of clearing the lung, relieving cough, detoxifying a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘红红张天柱刘俊张美丽杨蒙立
Owner 广西中农富玉国际农业科技有限公司
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