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Application of yap in the preparation of products for treating and/or preventing animal osteoarthritis

A product, aging technology, applied in the field of application in the preparation of products for the treatment and/or prevention of osteoarthritis in animals, which can solve problems such as the treatment of osteoarthritis

Active Publication Date: 2021-06-08
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no reports of targeting aging or longevity genes to treat osteoarthritis

Method used

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  • Application of yap in the preparation of products for treating and/or preventing animal osteoarthritis
  • Application of yap in the preparation of products for treating and/or preventing animal osteoarthritis
  • Application of yap in the preparation of products for treating and/or preventing animal osteoarthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1, YAP delays the aging of mesenchymal stem cells

[0128] 1.1 The expression of YAP decreases in the replicative senescence of mesenchymal stem cells

[0129] In the present invention, the human mesenchymal stem cells differentiated from human embryonic stem cells can be continuously passaged from the first generation to the twelfth generation under in vitro culture conditions by using the in vitro directed differentiation technology. The growth of mesenchymal stem cells at the 12th passage was completely arrested, and the cells became larger in size and flat in shape, showing a classic senescence phenotype. SA-β-gal staining results (attached figure 1 Middle A) shows that the SA-β-gal activity of the 12th passage mesenchymal stem cells is much higher than that of the 2nd passage mesenchymal stem cells.

[0130] The 2nd and 12th passage mesenchymal stem cells were used as the test cells, wherein the 2nd passage cells represent young cells, and the 12th passa...

Embodiment 2

[0153] Example 2. The therapeutic effect of YAP gene on osteoarthritis mice established by cutting the anterior cruciate ligament

[0154] Since the YAP-encoding lentivirus obtained in Example 1 has good properties of delaying the aging of mesenchymal stem cells in vitro, this example uses the YAP-encoding lentivirus obtained in Example 1 for osteoarthritis induced by anterior cruciate ligament cut Gene therapy in mice.

[0155] 2.1 Establish an osteoarthritis mouse model induced by anterior cruciate ligament cut

[0156] The mice used were all SPF grade, male, 10 weeks old, weighing 20±2g, and were purchased from Beijing Huafukang Biotechnology Co., Ltd. Experimental equipment and materials include: isoflurane gas anesthesia apparatus, epilator, surgical scissors, scalpel, Venus scissors, suture thread, penicillin, stereoscope, 20 μL micro-injector.

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Abstract

The invention discloses the application of YAP in preparing products for treating and / or preventing animal osteoarthritis. The present invention finds that YAP has the following functions: treating and / or preventing animal osteoarthritis; treating and / or preventing animal anterior cruciate ligament cut-induced osteoarthritis; delaying the aging of mesenchymal stem cells; delaying the replication of mesenchymal stem cells Sexual aging; Delaying pathological aging of mesenchymal stem cells. The experiment of the present invention proves that YAP has a definite function of delaying the aging of mesenchymal stem cells, and YAP has a definite therapeutic effect in osteoarthritis diseases. The invention provides a new idea for the development of gene therapy for osteoarthritis, and expands the optional range of clinical gene therapy.

Description

technical field [0001] The invention relates to the application of YAP in the preparation of products for treating and / or preventing animal osteoarthritis in the field of biomedicine. Background technique [0002] Osteoarthritis (OA) is a common degenerative disease characterized by the degeneration of articular cartilage, increased inflammation and the formation of osteophytes. Its clinical manifestations are joint pain, swelling, and dysfunction, which seriously affect the quality of life of patients. The occurrence of osteoarthritis is related to many factors, including genetics, age, gender, obesity, trauma, physical labor, endocrine, metabolism, immunity and so on. Among them, age is the most important pathogenic factor of osteoarthritis. With age, articular cartilage degenerates, the mucopolysaccharide content in the cartilage matrix decreases, the matrix loses chondroitin sulfate, the fiber component increases, and the toughness of the cartilage decreases. In addit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61P19/08A61P19/02A61P29/00A61P39/06
CPCA61K38/17A61P19/02A61P19/08A61P29/00A61P39/06
Inventor 曲静刘光慧付丽娜任若通
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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