BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold and preparation method and application thereof

A technology of strontium hydroxyapatite and BMP-2, which is applied in the fields of medical science, prosthesis, tissue regeneration, etc., to achieve the effect of easy availability of raw materials and simple preparation process

Active Publication Date: 2018-11-06
BEOGENE BIOTECH GUANGZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold loaded with BMP-2, which solves the short-term burst release of BMP-2 in the scaffold material problems, and improved the biological performance of existing hydroxyapatite-based scaffold materials

Method used

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  • BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold and preparation method and application thereof
  • BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold and preparation method and application thereof
  • BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0043] Example 1 Nano strontium-doped strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold loaded with BMP-2 and its preparation method

[0044] The preparation method of the BMP-2-loaded nano strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold includes the following steps:

[0045] (1) Take 8 g of silkworm cocoons without chrysalis and place them in 400 mL of deionized water containing 2 g of sodium bicarbonate, heat them to boiling, and after boiling for half an hour, take them out and wash them with deionized water, repeat the above operations until they are deionized with picric acid The carmine method qualitatively judges that silk fibroin no longer contains sericin. Then place the obtained silk fibroin in an oven at a temperature of 50° C., heat and dry overnight to remove moisture in the silk fibroin, and obtain the silk fibroin for use.

[0046] (2) Take 2.5g of pure silk fibroin and cut it into small pieces, put it in 25mL lithium...

Embodiment 2

[0054] Example 2 Hydroxyapatite / silk fibroin composite scaffold loaded with BMP-2 and its preparation method

[0055] The preparation method of the hydroxyapatite / silk fibroin composite scaffold loaded with BMP-2 of the present embodiment comprises the following steps:

[0056] (1) Take 8 g of silkworm cocoons without chrysalis and place them in 400 mL of deionized water containing 2 g of sodium bicarbonate, heat them to boiling, and after boiling for half an hour, take them out and wash them with deionized water, repeat the above operations until they are deionized with picric acid The carmine method qualitatively judges that silk fibroin no longer contains sericin. Then place the obtained silk fibroin in an oven at a temperature of 50° C., heat and dry overnight to remove moisture in the silk fibroin, and obtain the silk fibroin for use.

[0057] (2) Take 1 g of pure silk fibroin and cut it into small pieces, place it in 12.5 mL of lithium bromide solution with a concentrat...

Embodiment 3

[0060] Example 3 Nano strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold loaded with BMP-2 and its preparation method

[0061] The BMP-2-loaded nano strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold and the preparation method thereof in this embodiment include the following steps:

[0062] (1) Take 8 g of silkworm cocoons without chrysalis and place them in 400 mL of deionized water containing 2 g of sodium bicarbonate, heat them to boiling, and after boiling for half an hour, take them out and wash them with deionized water, repeat the above operations until they are deionized with picric acid The carmine method qualitatively judges that silk fibroin no longer contains sericin. Then place the obtained silk fibroin in an oven at a temperature of 50° C., heat and dry overnight to remove moisture in the silk fibroin, and obtain the silk fibroin for use.

[0063] (2) Take 2.5g of pure silk fibroin and cut it into small pieces, put it in 5...

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Abstract

The invention discloses a BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold and a preparation method and an application of the BMP-2 loaded strontium substituted hydroxyapatite/silk fibroin/heparin composite scaffold. The advantage that the silk fibroin and the strontium substituted hydroxyapatite are used in bone repair is taken; the heparin is combined onthe composite scaffold to slowly release a growth factor; the BMP-2 is then adsorbed, the growth factor is slowly released to promote the in vitro proliferation of a marrow stroma mesenchymal cell andinduce the marrow stroma mesenchymal cell to differentiate into a bone precursor cell, meanwhile, the doping of the strontium element effectively improves the bone induction capability, the mesenchymal stem cell in the body is collected to differentiate to a bone cell, and the composite scaffold is expected to be widely applied in the field of biological materials.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a BMP-2-doped strontium-doped hydroxyapatite / silk fibroin / heparin composite scaffold and its preparation method and application. Background technique [0002] Bone morphogenetic protein (BMP) is a multifunctional growth factor, among which BMP-2 is the most important regulatory factor in bone formation and promotes bone healing. Previous studies have found that: BMP-2 can promote the proliferation of bone marrow stromal mesenchymal cells in vitro and induce their differentiation into osteogenic precursor cells. Although BMP-2 has many advantages, it also has some disadvantages, such as: short-term burst release, easy inactivation, instability, short half-life and so on. [0003] Silk fibroin (SF) is a natural biological material extracted from silkworm cocoons. Because of its non-toxic, non-irritating, and good biocompatibility, the degradation is controllable throu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/50A61L27/46A61L27/20
CPCA61L27/20A61L27/46A61L27/50A61L27/54A61L27/56A61L2300/102A61L2300/252A61L2300/412A61L2300/45A61L2300/602A61L2430/02C08L89/00C08L5/10
Inventor 郭瑞蓝咏冯龙宝朱麒宇刘玉李丹毛宇
Owner BEOGENE BIOTECH GUANGZHOU
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