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Multi-channel three-dimensional microfluidic paper chip and preparation method thereof

A microfluidic paper chip, multi-channel technology, applied in chemical instruments and methods, analysis by chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc., can solve the problem of no research or patent reports. and other problems, to achieve the effect of expanding the detection linear range, low cost, and improving detection sensitivity

Active Publication Date: 2018-11-06
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although a variety of methods for the detection of the above-mentioned markers have been developed, there is no relevant research or research on a low-cost, portable paper-based detection method that can be used for the simultaneous high-throughput and high-sensitivity detection of the above-mentioned four targets. Patent report

Method used

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  • Multi-channel three-dimensional microfluidic paper chip and preparation method thereof
  • Multi-channel three-dimensional microfluidic paper chip and preparation method thereof
  • Multi-channel three-dimensional microfluidic paper chip and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The specific production, assembly and testing process is as follows:

[0039]1, at first utilize Computer Aided Design (CAD) drawing software to design paper chip model, this paper chip model has two sides, comprises upper layer filter paper A and lower layer filter paper B (such as figure 1 shown). The design parameters of the upper filter paper A and the lower filter paper B are as follows:

[0040] The upper layer of filter paper A has a sampling area 9 with a diameter of 10 mm. The sampling area 9 extends eight long flow channels in eight directions at the same angle. The width of the long flow channels is 3 mm and the length is 8 mm. Eight The extension ends of the long circulation channels are respectively provided with circular detection holes, and there is a hydrophobic gap of 2 mm between the extension ends of the long flow channels and the corresponding detection holes, and the diameter of the eight detection holes is 6 mm. The eight detection holes are numb...

Embodiment 2

[0059] In order to prove the beneficial effects of the present invention, the inventors used the paper chip of Example 1 to detect the concentrations of glucose, uric acid, cholesterol and choline in actual blood samples. The specific method is as follows:

[0060] "Computer Aided Design (CAD) drawing software" in embodiment 1 is changed into " Photoshop CS4 software "; " screen printing " in embodiment 1 is changed into " wax spray printing "; All the other are according to embodiment 1 step 1 , step 2 and step 3 to prepare a three-dimensional microfluidic paper chip for the simultaneous detection of glucose, uric acid, cholesterol and choline; add 120 μL of ultrafiltered serum sample dropwise to the central sampling area of ​​the paper chip, react for 10 minutes, and use Take a photo with the mobile phone to obtain the color picture, use Adobe Photoshop software to collect the gray value data of each detection area, and judge whether glucose, uric acid, cholesterol and cholin...

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Abstract

The invention discloses a multi-channel three-dimensional microfluidic paper chip and a preparation method thereof. The multi-channel three-dimensional microfluidic paper chip uses a piece of upper layer filter paper A and a piece of lower layer filter paper B printed with different microfluidic channels to form a three-dimensional microfluidic channel system through corresponding overlapping. Themulti-channel microfluidic paper chip provided by the invention realizes the simultaneous fast and sensitive determination of different substances, and can be used in the fields of biochemical analysis, immunoassay, sensors, instant detection device study and the like.

Description

technical field [0001] The invention relates to a multi-channel three-dimensional microfluidic paper chip and a preparation method thereof, belonging to the technical field of microfluidic paper chip production. Background technique [0002] The Whitesides research group first proposed the concept of paper chips in 2007. Paper chips use paper as a reaction carrier on the surface of paper through various methods, mainly including photolithography, inkjet printing, paraffin printing, and screen printing. , drawing method, laser processing method and cutting method, etc., to construct corresponding hydrophilic and hydrophobic regions. The sample and reaction solution are driven by the capillary force of the paper to realize the directional flow of the liquid in the hydrophilic channel, combined with a specific detection reaction, and finally realize a new type of paper chip detection that integrates a series of operations such as sample injection, reaction and detection. techn...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/78
CPCB01L3/5027B01L2200/10G01N21/78
Inventor 李芳刘家昌王旭何建波
Owner HEFEI UNIV OF TECH
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