Cellulose degrading bacteria compound system

A technology of cellulose-degrading bacteria and sodium carboxymethyl cellulose, applied in the field of microorganisms, can solve the problems of cellulose-degrading effects, inactivation of degrading bacteria, unfavorable combination of bacteria, etc., achieve optimal cellulose-degrading function, eliminate antagonism, Effect of improving cellulose degradation efficiency

Inactive Publication Date: 2018-11-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the actual use of microbial agents, due to the influence of biotic and abiotic factors in the surrounding environment, the adaptability and competitiveness of microorganisms often change in complex environments. The function of degrading cellulose under low temperature and alkaline conditions is affected, etc.
In order to enhance the effect of microorganisms in composting, many scholars and experts have developed composite microbial agents. However, the composting fermentation process of solid organic matter is an extremely complicated process of microbial action, and temperature and pH are important factors affecting microbial growth. Existing No cellulose-degrading bacteria complex line with high stability and high degradation rate has been cultivated in the technology, and the combination of cellulose-degrading bacteria complex lines needs to be screened. Random combinations are not necessarily beneficial to the growth of strains, and may directly Inhibiting its growth will have an adverse effect on the function of the combined bacteria, and it is not convenient for the mixed fermentation culture of the complex bacteria

Method used

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  • Cellulose degrading bacteria compound system
  • Cellulose degrading bacteria compound system
  • Cellulose degrading bacteria compound system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A compound strain of cellulose-degrading bacteria, including Alternaria, Streptomyces, Erchetia and Aureobasidium, the ratio of which is 1:1:1:1.

[0026] The preparation method of the above-mentioned cellulose-degrading bacteria composite system comprises the following steps:

[0027] (1) Extraction of complex bacterial strains: Collect humus from Yu'an Mountain in the western suburbs of Kunming, bottom mud of Dianchi Lake in Kunming, excrement around toilets, and cow dung in Dali, and isolate Alternaria, Streptomyces, Erchetia and Brachyphyllum from them After mold, store at low temperature;

[0028] (2) Preparation of medium: 3 g of peptone, 2 g of ammonium sulfate, 0.5 g of yeast extract, KH 2 PO 4 4g, CaCl 2 ·2H 2 O0.3g, MgSO 4 ·7H 2 O 0.3g, Tween-800.2mL, CMC 20g, distilled water 1000mL, after mixing evenly, sterilize at 125°C for 15min to obtain carboxymethylcellulose sodium liquid fermentation medium.

[0029] (3) The four kinds of bacterial strains are ...

Embodiment 2

[0032] A compound strain of cellulose-degrading bacteria, including Alternaria, Streptomyces, Erchetia and Aureobasidium, the ratio of which is 1:1:1:1.

[0033] The preparation method of the above-mentioned cellulose-degrading bacteria composite system comprises the following steps:

[0034] (1) Extraction of complex bacterial strains: Collect humus from Yu'an Mountain in the western suburbs of Kunming, bottom mud of Dianchi Lake in Kunming, excrement around toilets, and cow dung in Dali, and isolate Alternaria, Streptomyces, Erchetia and Brachyphyllum from them After mold, store at low temperature;

[0035] (2) Preparation of medium: 3 g of peptone, 2 g of ammonium sulfate, 0.5 g of yeast extract, KH 2 PO 4 4g, CaCl 2 ·2H 2 O0.3g, MgSO 4 ·7H 2 O 0.3g, Tween-800.2mL, CMC 20g, distilled water 1000mL, after mixing evenly, sterilize at 115°C for 25min to obtain carboxymethylcellulose sodium liquid fermentation medium.

[0036] (3) The four kinds of bacterial strains are ...

Embodiment 3

[0039] A compound strain of cellulose-degrading bacteria, including Alternaria, Streptomyces, Erchetia and Aureobasidium, the ratio of which is 1:1:1:1.

[0040] The preparation method of the above-mentioned cellulose-degrading bacteria composite system comprises the following steps:

[0041] (1) Extraction of complex bacterial strains: Collect humus from Yu'an Mountain in the western suburbs of Kunming, bottom mud of Dianchi Lake in Kunming, excrement around toilets, and cow dung in Dali, and isolate Alternaria, Streptomyces, Erchetia and Brachyphyllum from them After mold, store at low temperature;

[0042] (2) Preparation of medium: 3 g of peptone, 2 g of ammonium sulfate, 0.5 g of yeast extract, KH 2 PO 4 4g, CaCl 2 ·2H 2 O0.3g, MgSO 4 ·7H 2 O 0.3g, Tween-800.2mL, CMC 20g, distilled water 1000mL, after mixing evenly, sterilize at 120°C for 20min to obtain carboxymethylcellulose sodium liquid fermentation medium.

[0043] (3) The four kinds of bacterial strains are ...

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Abstract

The invention provides a cellulose degrading bacteria compound system and relates to the field of microorganisms. The compound system comprises alternaria, streptomycete, oerskovia and aureobasidium pullulans according to a proportion of 1:1:1:1. The compound system presents high cellulose enzyme activity function stability within a temperature range of 35-55 DEG C and a pH range of pH 6-10 and can normally decompose cellulose.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a composite system of cellulose-degrading bacteria. Background technique [0002] Agricultural organic waste is one of the key points and hotspots in the field of environmental protection in my country. Using composting technology to effectively utilize agricultural organic waste is an important means to realize the harmless and resourceful use of agricultural organic solid waste, and it is also an important means to protect the agricultural ecological environment. , An important measure to reduce environmental pollution and waste of resources caused by improper use of agricultural organic waste. The role of microorganisms in composting is very important. Many studies have found that microorganisms can promote and optimize the composting process, increase the content of nutrients such as total nitrogen, available phosphorus and available potassium, and improve the quality of compost....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C05F17/00C12R1/645C12R1/465
CPCC05F11/08C05F17/20C12N1/14C12N1/20Y02W30/40
Inventor 包立董新星肖艳萍张乃明刘惠见邓洪
Owner YUNNAN AGRICULTURAL UNIVERSITY
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