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Microfluidic aliquoting for single-cell isolation

A single-cell separation and microfluidic technology, applied in the field of single-cell separation, can solve problems such as poor compatibility and limited application, and achieve the effects of small sample loss, simple operation, and simple operation experience requirements.

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the comparable size of microchannels and cells, recently developed microfluidics technology provides an important avenue for single-cell manipulation; however, its potential disadvantages, such as the need for additional microfluidic manipulation skills, poor compatibility with existing experimental platforms, and inability / difficulty to selectively retrieve isolated single cells from microchips for further analysis, greatly limiting their use in general laboratories

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  • Microfluidic aliquoting for single-cell isolation
  • Microfluidic aliquoting for single-cell isolation
  • Microfluidic aliquoting for single-cell isolation

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Embodiment Construction

[0056] At times, the invention has been described herein in terms of example environments. Descriptions of these environments are provided to allow various features and embodiments of the invention to be depicted in the context of exemplary applications. After reading this specification it will become apparent to a person of ordinary skill in the art how to implement the invention in different and alternative environments.

[0057] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0058] All patents, applications, published applications and other publications mentioned herein are incorporated by reference in their entirety. To the extent that definitions set forth in this section are contrary to or inconsistent with definitions set forth in applications, published applications, and other publications incorporated herein by reference, the d...

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Abstract

According to the invention, generally, a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by a plurality of microchannels to a plurality of side wells (outlets). A relatively large (such as 120 muL) cell suspension having several cells may be injected into the inlet of the MA chip, and single cells may be substantially simultaneously and uniformly distributed, via positive pressure-driving flow, to the several (such as 120) side wells having single cells in less than 1 minute. The MA Chip has a high efficiency in cell recovery. Due to rapidisolation and easy identification of single cells, high cell viability, high enrichment factor, and convenient transfer of submicroliter single-cell suspension, MA Chips are well compatible with CTCisolation from blood, single-cell cloning, PCR, and sequencing.

Description

technical field [0001] In some embodiments of the present invention, the present invention broadly relates to the field of single cell separation methods and devices, in particular, to a technique for separating single cells using microfluidic technology, in particular, to a single cell separation Microfluidic aliquot chip. Background technique [0002] Microfluidics is an interdisciplinary field of engineering involving physics, chemistry, biochemistry, nanotechnology, and biotechnology that puts system design into practice, handling small amounts of fluid for multiplexing, automation, and high-efficiency. Throughput screening. Advances in microfluidics are revolutionizing molecular biology procedures for enzyme analysis (e.g., glucose and lactate assays), DNA analysis (e.g., polymerase chain reaction and high-throughput sequencing) and proteomics, microfluidic biochips The basic idea is to integrate detection operations, such as detection, as well as sample pretreatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/20B01L3/00C12Q1/02
CPCB01J2219/00317B01J2219/00421B01J2219/00536B01J2219/00743B01L3/502761B01L2200/0668B01L2300/021B01L2300/0803B01L2300/0864B01L2400/0457C12M47/04B01L2200/0652B01L2200/10B01L2300/0806B01L2300/0861B01L2300/123C12Q1/04B01L3/5023B01L3/5027B01L2300/0893B01L2400/0487
Inventor 秦立东张凯
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