Method used for identifying amino acid enantiomers through cyclodextrin nanoparticle electrochemistry

A nano-microsphere, electrochemical technology, applied in the field of molecular recognition, can solve the problems of limited popularization, strict condition control, result stability and reproducibility dependence, etc., and achieve good electrochemical recognition effect and high stability effect

Inactive Publication Date: 2018-11-13
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned modified electrode obtained a better recognition effect of tryptophan enantiomers, but the self-assembly process of the electrode preparation process took a long time, the conditions were strictly controlled, and the stability and reproducibility of the results depended on the technical proficiency of the experimental operator, which limited this method. popularization of technology

Method used

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  • Method used for identifying amino acid enantiomers through cyclodextrin nanoparticle electrochemistry
  • Method used for identifying amino acid enantiomers through cyclodextrin nanoparticle electrochemistry
  • Method used for identifying amino acid enantiomers through cyclodextrin nanoparticle electrochemistry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Weigh 10 mg nanosphere NCC-CDP, dissolve it in 5 mL ultrapure water, and ultrasonically disperse it for 30 min;

[0017] (2) Pipette 5 μL of dispersion liquid evenly drop-coated on the surface of 3mm glassy carbon electrode, and let it dry naturally to prepare NCC-CDP modified electrode;

[0018] (3) Prepare 100 mL of L-Trp and D-Trp solutions with a concentration of 0.5 mM respectively, the solvent is 0.1 M PBS, pH=7;

[0019] (4) First, immerse the modified electrode in the L-Trp solution for differential pulse voltammetry scanning (electrochemical window 0.4-1.2V), and then use cyclic voltammetry (sweep rate 0.1V / s, cycle number 20) to scan to Restore the activity of the modified electrode, and finally immerse the modified electrode in the D-Trp solution for differential pulse voltammetry scanning, record the potential-current response signal of the modified electrode, and perform electrochemical experiments on the tryptophan enantiomers between 5 and 40°C. To i...

Embodiment 2

[0021] (1) Weigh 10 mg nanosphere NCC-CDP, dissolve it in 5 mL ultrapure water, and ultrasonically disperse it for 30 min;

[0022] (2) Use a pipette gun to pipette 5 μL of the dispersion evenly onto the surface of a 3 mm glassy carbon electrode, and let it dry naturally to obtain Cu-containing 2+ Nano-microsphere modified electrode;

[0023] (3) Prepare 100mL of L-Trp and D-Trp solutions with a concentration of 0.5mM respectively, the solvent is 0.1M PBS, pH=7, add 1.35g of CuCl 2 , introducing Cu 2+ ;

[0024] (4) At a temperature of 25°C, first immerse the modified electrode in the L-Trp solution for differential pulse voltammetry scanning (electrochemical window 0.4-1.2V), and then use cyclic voltammetry (scan rate 0.1V / s, Segments 40) scan to restore the activity of the modified electrode, and finally immerse the modified electrode in the D-Trp solution for differential pulse voltammetry scanning, record the potential-current response signal of the modified electrode, ...

Embodiment 3

[0026] (1) Weigh 10 mg nanosphere NCC-CDP, dissolve it in 5 mL ultrapure water, and ultrasonically disperse it for 30 min;

[0027] (2) Use a pipette gun to pipette 5 μL of the dispersion evenly onto the surface of a 3 mm glassy carbon electrode, and let it dry naturally to obtain an NCC-CDP modified electrode;

[0028] (3) Prepare 100 mL of tyrosine Tyr (or phenylalanine Phe) enantiomer solutions with a concentration of 0.5 mM respectively, the solvent is 0.1 M PBS, pH=7;

[0029] (4) At a temperature of 25°C, first immerse the modified electrode in L-Tyr (or L-Phe) solution for differential pulse voltammetry scanning (electrochemical window 0.4-1.2V), and then use cyclic voltammetry (scanning Speed ​​0.1V / s, number of segments 40) to restore the activity of the modified electrode, and finally immerse the modified electrode in D-Tyr (or D-Phe) solution for differential pulse voltammetry scanning, record the potential-current response signal of the modified electrode, and carr...

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Abstract

The invention provides a method used for identifying amino acid enantiomers through cyclodextrin nanoparticle electrochemistry. The method comprises following steps: cellulose nanometer crystal, beta-cyclodextrin, and a tetrafluoroterephthalonitrile terpolymer (NCC-CDP) nanoparticle are used for dropping coating of the surface of a glassy carbon electrode so as to obtain a modified electrode; anddifferential pulse voltammetry is adopted for selective identification of tryptophan enantiomers. It is shown that the tryptophan enantiomers can be identified easily, when Cu<2+> is added into tryptophan, identification rate is increased further, and at the same time, the modified electrode possesses identification effect on tyrosine and phenylalanine enantiomers.

Description

technical field [0001] The invention belongs to the field of molecular recognition, and in particular relates to a chiral material recognition method based on a nano-microsphere modified electrode. Background technique [0002] Chirality is an inherent property of nature. Chiral compounds have consistent physical and chemical properties and are difficult to distinguish. Currently, common methods for chiral recognition include chiral column high performance liquid chromatography, capillary electrophoresis, and electrochemical chiral sensors. Electrochemical identification methods have received great attention due to low cost and intuitive and understandable signals. [0003] We have reported electrochemical chiral sensors based on β-cyclodextrin and its derivatives. Including: a chiral sensor based on β-cyclodextrin and its preparation method, ZL2014 1 0579838.0; a chitosan / cyclodextrin binuclear copper-based chiral sensor and its preparation method, ZL 2012 1 0197747.5. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 陶永新冯永强吕泳其彭勇刚秦勇孔泳
Owner CHANGZHOU UNIV
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