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Preparation and detection method of a water-soluble dimethyl curcumin liposome fluorescent probe

A technology of dimethyl curcumin lipid and fluorescent probe, which is applied in the field of fluorescent probes, can solve the problems of fluorescence quenching detection and performance of water-soluble dimethyl curcumin liposome fluorescent probes that have not been reported, and achieve Good selectivity and anti-interference, simple detection method, easy to control the effect of preparation conditions

Active Publication Date: 2020-10-09
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the water-soluble dimethyl curcumin liposome fluorescent probe prepared by the present invention has not yet been reported in terms of fluorescence quenching detection and performance, etc.

Method used

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  • Preparation and detection method of a water-soluble dimethyl curcumin liposome fluorescent probe
  • Preparation and detection method of a water-soluble dimethyl curcumin liposome fluorescent probe
  • Preparation and detection method of a water-soluble dimethyl curcumin liposome fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of ASC-J9 liposomes:

[0032] (1) ASC-J9 liposomes were prepared by film dispersion method. Add natural phospholipids (1000mg), cholesterol (100mg), 10mg of dimethyl curcumin powder in turn to the round bottom flask, add 100mL of absolute ethanol to dissolve, 60 ℃ vacuum rotary steaming, then add 150mL of H 2 O, continue to react at 60°C for 30min, and continue to incubate in a water bath at 60°C for 3h until a suspension is formed.

[0033] (2) Ultrasonic disperse the ASC-J9 liposomes hatched in (1), ultracentrifuge (8000rpm, 45min) after ultrasonic dispersion for 15 minutes, take the upper layer of liquid as ASC-J9 liposomes, and measure the encapsulation efficiency 97%

Embodiment 2

[0035] With reference to the method of Example 1, the encapsulation efficiency obtained by adding acetonitrile to dissolve raw materials in step (1) was 95%.

Embodiment 3

[0037]Referring to the method of Example 1, step (1) natural phospholipids, cholesterol and ASC-J9 participated in the reaction at a mass ratio of 1000:100:30, and the encapsulation efficiency of ASC-J9 liposome was 82%.

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Abstract

The invention belongs to the field of fluorescent probes, particularly relates to preparation of a fluorescent probe for detecting Fe<3+> and a detection method of the fluorescent probe, and specifically relates to dimethyl curcumin liposome. The preparation process is simple, the reaction conditions are easy to control, and the prepared fluorescent probe has excellent optical performance and optical stability; and it is found after Fe<3+> is detected by adopting a fluorescence quenching method that the prepared fluorescent probe has good Fe<3+> recognition performance and strong anti-interference ability, and can be applied to analysis and detection of Fe<3+> in a pure water medium.

Description

technical field [0001] The invention belongs to the field of fluorescent probes, in particular to the preparation and detection method of a water-soluble dimethyl curcumin liposome fluorescent probe. Background technique [0002] As an essential trace element in the life system, iron plays a vital role in many physiological and pathological processes such as protein synthesis, enzymatic conversion, oxygen transport and cell metabolism. Too much or too little Fe 3+ Both will interfere with the homeostasis of cells, leading to the occurrence of various diseases such as anemia, Alzheimer's disease, diabetes, Parkinson's, etc. Therefore, Fe 3+ The detection of these diseases has far-reaching significance for the early prevention, diagnosis and treatment of these diseases. Commonly used to detect Fe 3+ The most common methods include atomic absorption spectrometry, inductively coupled plasma spectrometry, and voltammetry, but these instruments are complex to operate and costly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/06G01N21/64
CPCC09K11/025C09K11/06C09K2211/1007G01N21/6428G01N2021/6432
Inventor 李正义邵丹迪殷乐肖唐鑫孙小强
Owner CHANGZHOU UNIV