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Method for simultaneously determining contents of three fat-soluble components in red sage root-pseudoginseng preparation

A technology of preparation and usage, applied in the field of simultaneous determination of three fat-soluble components in Danqi preparation, can solve the problems of limited research on lipophilic components, avoid frequent replacement of detection conditions, and improve work efficiency

Inactive Publication Date: 2018-11-16
BEIJING RED SUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on Danqi preparations mainly focuses on the hydrophilic salvianolic acid and saponin components, while the research on lipophilic components is quite limited.

Method used

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  • Method for simultaneously determining contents of three fat-soluble components in red sage root-pseudoginseng preparation
  • Method for simultaneously determining contents of three fat-soluble components in red sage root-pseudoginseng preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Detection of campesterol, stigmasterol and β-sitosterol content in Danqi preparation

[0017] (1) Instrument

[0018] TRACE 1310 / TSQ 8000 gas chromatography-mass spectrometry (Thermo, USA), SB-800DTD ultrasonic cleaner (Ningbo Xinzhi Biotechnology Co., Ltd.), analytical balance (BSA224S, Sartorius), 0.22 μm PTFE membrane ( Tianjin Jinteng Experimental Equipment Co., Ltd).

[0019] (2) Reagents and samples

[0020] The reference substances campesterol (purity ≥ 95%), stigmasterol (purity ≥ 95%), β-sitosterol (purity ≥ 95%) and 5α-cholestane were purchased from Sigma Corporation of the United States; BSTFA (bistrifluoroacetamide , containing 1% TMCS (trimethylchlorosilane)) (Supelco); dichloromethane, n-hexane, ethyl acetate, 95% ethanol, concentrated hydrochloric acid, KOH (AR, Sinopharm Chemical Reagent Co., Ltd.). Danqi Tablets (Beijing Tongrentang Technology Development Co., Ltd. Pharmaceutical Factory, batch numbers: 14120431, 14120810, 14120906, 141221...

Embodiment 2

[0041] Determination of campesterol, stigmasterol and β-sitosterol content in embodiment 2 Danqi preparation

[0042] Danqi Tablets, Danqi Soft Capsules and Compound Danshen Dripping Pills of different batch numbers were prepared according to the method of Example 1 for the test solution, and GC-MS analysis was carried out to measure β-sitosterol, stigmasterol and The content of campesterol, the results are shown in Table 1.

[0043] Table 1 Contents of sterols in different Danqi prescriptions (n=3)

[0044]

[0045] The present invention adopts gas chromatography-mass spectrometry (GC-MS) to measure the contents of three fat-soluble components in different Danqi prescription medicines-beta-sitosterol, stigmasterol and campesterol. The method has high accuracy, good reproducibility, and strong applicability. Through retention time and mass spectrometry, the content of these three fat-soluble components in different types of drugs can be accurately determined. It can be se...

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Abstract

The invention specifically relates to a method for simultaneously determining the contents of three fat-soluble components in a red sage root-pseudoginseng preparation, belonging to the technical field of medicine. The method employs combined gas chromatography mass spectrometry to simultaneously determine the contents of the three fat-soluble components consisting of campesterol, stigmasterol andbeta-sitosterol in the red sage root-pseudoginseng preparation. Experimental results prove that the method of the invention is stable and reliable, and can provide an important basis for the qualityevaluation of the red sage root-pseudoginseng preparation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for simultaneously measuring the contents of three fat-soluble components in Danqi preparations. Background technique [0002] The whole prescription of Danqi Preparation is composed of Danshen and Panax notoginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain; the combined use of the two medicines has the effect of promoting blood circulation, dissipating blood stasis, reducing swelling and relieving pain. Clinically, it is mostly used for diseases caused by congestion, such as: chest obstruction and heartache, congestion headache, bruises, irregular menstruation, lower abdominal pain caused by postpartum stasis, traumatic hematoma, dysmenorrhea, lochia, coronary heart disease, angina pectoris, high blood pressure etc. In addition to the earliest Danqi tablets, the types of Danqi preparations also a...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 林德良杨健
Owner BEIJING RED SUN PHARMA