A kind of laver antihypertensive peptide and laver antihypertensive peptide extract and application
A technology of antihypertensive peptide and seaweed, applied in the direction of application, peptide, drug combination, etc.
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Embodiment 1
[0023] (1) Take 40g of seaweed powder, add 1200ml of water to soak at room temperature for 3 hours, add 800mg of trypsin, stir and control the temperature at 40°C during enzymolysis, adjust the pH of the solution to 7.8, after 3 hours of enzymolysis, inactivate the enzyme at 100°C for 10 minutes .
[0024] (2) Impurities were removed by filtration, the supernatant was precipitated with ethanol with a final volume concentration of 75%, the precipitate was removed by centrifugation, the supernatant was concentrated to 20 ml, and freeze-dried.
[0025] (3) The components obtained by alcohol precipitation were further separated and purified using Sephadex G-25, distilled water was used as the mobile phase, and the flow rate was controlled at 0.8ml / min. The components with a retention time of 35min-36min were collected and concentrated to freeze-dry.
[0026] (4) The above chromatographically purified components were identified using UPLC-ESI-Q-Exactive Focus-MS / MS. Mascot2.2 compa...
Embodiment 2
[0028] (1) Take 40g of seaweed powder, add 1600ml of water to soak at room temperature for 2h, adjust the pH to 8.0, and the temperature at 37°C, add 1200mg of trypsin to start the enzymolysis reaction, after 2h, boil water for 10min to terminate the reaction.
[0029] (2) Remove impurities by filtration, use ethanol with a final volume concentration of 80% to precipitate impurities, remove impurities by filtration, concentrate to 20 ml and lyophilize.
[0030] (3) The above components were separated and purified by Sephadex G-25 gel chromatography, the mobile phase was distilled water, the flow rate was 0.8ml / min, the components with a retention time of 35min-36min were collected, concentrated and freeze-dried.
[0031] (4) Use UPLC-ESI-Q-Exactive Focus-MS / MS to identify the collected components. Mascot2.2 compares the mass spectrum information with the Porphyra transcriptome (SRX1016678, downloaded to NCBI), and selects the top 20 and Polypeptides with polar or large group r...
Embodiment 3
[0033] The sequence of the polypeptide is identified above, the artificially synthesized polypeptide TGAPCR with a purity >98% is described below, and the physicochemical properties and antihypertensive activity in vivo of TGAPCR are evaluated below.
[0034] (1) Determination of ACE inhibitory activity of peptides in vitro by high performance liquid chromatography (HPLC)
[0035] The medicines and instruments used in this example can be obtained commercially without special instructions.
[0036] The principle of high performance liquid chromatography (HPLC) assaying ACE inhibitory activity is: ACE can hydrolyze the substrate hippuryl-histidyl-leucine (Hip-His-Leu, HHL, Sigma Company of the United States) into hippuric acid, When an enzyme inhibitor is added, the amount of hippuric acid generated is correspondingly reduced, and the inhibition rate of the inhibitor on ACE can be determined by detecting the absorption peak area of hippuric acid at an ultraviolet wavelength of...
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