A kind of peach gum polysaccharide and preparation method thereof
A technology of peach gum polysaccharide and peach gum, which is applied in the field of peach gum polysaccharide and its preparation, can solve the problems of high degree of polymerization, poor water solubility, and low glucuronic acid content of peach gum, achieve good solubility, high yield, and benefit absorption effect
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[0028] The preparation method of peach gum polysaccharide comprises the steps:
[0029] 1. Add peach gum to soak in water, heat and boil for 4-6 hours, filter, and take the filtrate.
[0030] First, the peach gum is swelled in water, and the carbohydrates in the peach gum are continuously hydrolyzed in water to produce water-insoluble substances. At the same time, the polysaccharides and proteins in the peach gum enter the water, and after filtration, the obtained filtrate mainly contains peach gum Polysaccharides, proteins and water etc.
[0031] Optionally, the mass ratio of water to peach gum is 1: (80-120), so that the swelling and hydrolysis effects of peach gum are good, so that more peach gum polysaccharides can be separated later, and the yield of peach gum polysaccharides can be increased.
[0032] Soaking is carried out at 60-80°C, so that the swelling and hydrolysis effect of peach gum is better, so that more peach gum polysaccharides can be separated later, and th...
Embodiment 1
[0049] A preparation method of peach gum polysaccharide, comprising the steps of:
[0050] (1), adding peach gum to water for soaking, heating and boiling for 5 hours, filtering, and taking the filtrate.
[0051] (2), add dichloromethane and normal hexane mixed solvent in filtrate, separate liquid, take supernatant.
[0052] (3) Mixing and extracting the supernatant liquid with alcohols to obtain peach gum polysaccharide.
Embodiment 2
[0054] A preparation method of peach gum polysaccharide, comprising the steps of:
[0055] (1) First, grind the original peach gum into peach gum powder, sieve to remove large particles of impurities, add 1 mass part of peach gum to 110 mass parts of distilled water, and put it in a constant temperature oven with a temperature of 70°C for soaking After heating and boiling for 5 hours, filter with gauze and take the filtrate. The filtrate was concentrated in a rotary evaporator to obtain a concentrated filtrate.
[0056] (2), dichloromethane and n-hexane mixed solvent are added to the concentrated filtrate to mix the two, separate liquids through a separatory funnel, and repeat several times until the middle layer has no protein layer, and the upper layer is taken through the second stage of the rotary evaporator. After the secondary concentration, the concentrated supernatant was obtained, and hydrogen peroxide with a volume fraction of 30% was added for decolorization to obtai...
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