Bacillus licheniformis fermentation technology, and application thereof to preparation of microbial agent and biological organic fertilizer for preventing cucumber gray mold

Abstract

The invention discloses a bacillus licheniformis fermentation technology, and application thereof to preparation of a microbial agent and biological organic fertilizer for preventing cucumber gray mold. Manganese sulfate can be slowly released into a culture medium under the supporting of broken egg shells and newspaper scraps, is uniformly distributed and obvious in effect, can obviously improvethe spore conversion speed with the matching of relevant technologies, the culture tie is shortened, and the cost is reduced; muskmelon base leachate has toxicity, can not only eliminate the toxicityand ensure that infectious microbe does not exist with the matching of relevant technologies, but also release nutritional ingredients, such as glucose, galactose and amino acid, contained in muskmelon base, and bacillus licheniformis contained in formed biological organic fertilizer and microbial agent belongs to biocontrol bacterium, and can effectively prevent the cucumber gray mold. The biological organic fertilizer can promote the development of roots, ensure that cucumber plants are healthy, and enhance the gray mold resistance; the microbial agent sprayed to leaf surfaces can form a protective layer, prevent invasion of botrytis cinema, and achieve protection effect on cucumber leaves.

Description

technical field [0001] The invention relates to a Bacillus licheniformis fermentation process and a method for preventing gray mold of cucumber. The preservation number of the strain is CGMCC1.813. The strain is a biocontrol bacterium, which has obvious inhibitory effect on Botrytis cinerea. The invention uses a bacillus licheniformis as a functional bacterium to prepare a bio-organic fertilizer and a microbial agent, which has a preventive effect on cucumber gray mold. Background technique [0002] Botrytis cinerea is a disease that occurs perennially in protected cultivation of cucumbers, and it has become more serious in recent years. Because the fruit is often infested and causes rot, it is often called fruit rot disease and mildew rot disease. The disease is caused by the infection of Botrytis cinerea Pers.ex Fr. Overwinter or oversummer with sclerotia in soil or on diseased residues with mycelium and conidia. The conditions in the following spring are suitable, the...

AI Technical Summary

Problems solved by technology

The traditional method of treating this disease is to spray the infected parts with pesticides, resulting in pesticide residues, environmental pollution, and endangering human health.

And the traditional use of large chemical fertilizers makes the soil increasingly poor, resulting in underdeveloped cucumber roots, weak growth and weak disease resistance

The focus of Botrytis cinerea is on prevention. Infection will inevitably lead to a decline in the yield and quality of cucumbers, affecting the income of vegetable farmers

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