Method for detecting contents of various preservatives in solid food
A detection method and technology for preservatives, which are used in the detection of various preservatives, can solve the problems of false negatives, high requirements for gas chromatography-mass spectrometry instruments, and false positives.
Inactive Publication Date: 2018-11-20
肇庆市创业帮信息技术有限公司
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- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Although liquid chromatography and liquid chromatography-mass spectrometry can simultaneously determine a variety of preservatives, due to the variety of food sample matrices, the matrix impurities will cause interference, which may easily cause false positive or false negative results, affecting accurate quantification
Gas chromatography-mass spectrometry has high requirements for instruments and is not suitable for popularization
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0022] A method for detecting the content of various preservatives in solid food, comprising the following steps:
[0023] (1) Take 5.00g of the sample to be tested and crush it into powder, add the sample to be tested into a saturated sodium chloride solution and place it in an ultrasonic oscillator for 5 minutes, add dilute nitric acid to adjust the pH to 4-5, and precipitate the protein , filtered to remove insoluble matter;
[0024] (2) adding normal hexane in the filtrate to carry out sub-extraction, and separate the fat;
[0025] (3) Add adsorbent in the extraction residual liquid, the adsorbent is a mixture of carbon nanofibers, amino silica gel and graphene oxide with a weight ratio of 1:2:6, and the ratio of the adsorbent addition to the extraction residual liquid is 0.3g / L, shake for 40min, then centrifuge, take the adsorbent, analyze with ethanol ultrasonic vibration analysis to obtain the analysis solution, the ratio of adsorbent and ethanol addition is 2g / L;
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Abstract
The invention belongs to the technical field of food detection, and particularly relates to a method for detecting the contents of various preservatives in a solid food. The method comprises the following steps: mashing a sample to be tested, precipitating and removing protein with a saturated sodium chloride aqueous solution, carrying out extraction to remove fat components by using n-hexane, adding an adsorbent, carrying out vibrating and centrifuging, analyzing the adsorbent with ethanol, carrying out drying, using acetone for fixing the volume, carrying out filtration with a filter membrane, and carrying out detection by using a gas chromatograph equipped with a hydrogen flame detector. According to the method provided by the invention, interference components in the sample to be detected can be effectively removed by means of protein precipitation, n-hexane extraction of fat components and further purification by an adsorbent, and 12 preservatives in the sample to be detected canbe simultaneously detected.
Description
technical field [0001] The invention belongs to the technical field of preservative detection, and in particular relates to a method for detecting the content of various preservatives in solid food. Background technique [0002] Preservative refers to the agent that inhibits the corruption of substances, that is, it has a continuous inhibitory effect on the growth of microorganisms that use spoilage substances as metabolic substrates, and is widely used in food, daily chemical and other fields. There are many types of preservatives that are allowed to be added internationally, such as about 50 in the United States and 40 in Japan. At present, my country has only approved 32 kinds of food preservatives that are allowed to be used. They have undergone a lot of scientific experiments before being approved for use. They are all low-toxic and safe varieties, mainly including: benzoic acid and its sodium salt, Sorbic acid and its potassium salt, sulfur dioxide, sodium (potassium) ...
Claims
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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 贺士友刘海军
Owner 肇庆市创业帮信息技术有限公司