Use of ascophyllum nodosum extracts for regulating expression of gene groups
A technology of Ascophyllum nodosum and extract, which is applied in the direction of drug combination, application, food science, etc., and can solve problems such as high cost, limited real effect, and adverse skin effects
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Embodiment 1
[0036] Preparation of Ascophyllum nodosum extract
[0037] Firstly, wash the Ascophyllum nodosum, and process it into an appropriate size by, for example, cutting and grinding, and then extract the treated Ascophyllum nodosum with water, alcohol, or a mixture of alcohol and water. The solvent is preferably water, and the liquid-solid ratio of the solvent to the Ascophyllum nodosum is 5-20:1-5. The extraction temperature is between 50°C to 100°C, preferably 80°C to 95°C. The extraction time in this embodiment is 0.5-3 hours.
[0038] After the Ascophyllum nodosum extract obtained through the above extraction steps is cooled to room temperature, it can be filtered through a 400 mesh filter to remove residual solids. The filtered Ascophyllum nodosum extract can be further concentrated under reduced pressure at 45° C.˜70° C. to obtain a concentrated product. In order to obtain the solid Ascophyllum nodosum extract, the aforementioned Ascophyllum nodosum extract concentrated und...
Embodiment 2
[0040] Ascophyllum nodosum extract enhances the expression of specific gene groups in epidermal keratinocytes
[0041] In order to explore the regulatory effect of Ascophyllum nodosum extract on gene expression in skin cells, in this example, qPCR technology was used to measure the gene expression changes of primary human epidermal keratinocytes HPEK-50 treated with water extract of Ascophyllum nodosum. First, the 1.5×10 5 HPEK-50 cells were inoculated into each well of a 6-well plate containing 2 mL of cell culture medium and cultured at 37°C. Secondly, these cells were treated with 2 mL of SFM medium containing 1 mg / mL Ascophyllum nodosum water extract (triple experiment), which was used as the experimental group. At the same time, another group of HPEK-50 cells treated with SFM medium without Ascophyllum nodosum water extract was set as a control group. After 6 or 24 hours, the cells were harvested for qPCR.
[0042] The relative expression levels of genes such as TGM1, ...
Embodiment 3
[0044] Extract of Ascophyllum nodosum enhances the resistance of dermal fibroblasts to ultraviolet radiation
[0045] To examine whether Ascophyllum nodosum extract affects skin resistance to UV irradiation, the cell survival of human dermal fibroblasts CCD-966SK irradiated with UVA after treatment with Ascophyllum nodosum extract was assessed using a cell survival assay (MTT assay) Rate. In short, the 5×10 3 CCD-966SK cells were inoculated into each well of a 96-well plate containing 200 μL of cell culture medium. After culturing at 37°C for 24 hours, the cell medium was removed and 200 μL of cells containing 1 mg / mL Ascophyllum nodosum water extract was added. Medium to cells were used as experimental groups, which were further cultured at 37°C for 24 hours. Thereafter, the cells were subjected to 12 J / cm in a UV irradiation box (Vilber) 2 Ultraviolet A (wavelength 315-400nm) is irradiated for 1 hour, and this radiation dose will cause half of the cells to die. At the sa...
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