Kit and system for predicting susceptibility of laryngeal squamous cell carcinoma

A squamous cell carcinoma, susceptibility technology, applied in the field of biomedicine, can solve the impossible, impossible early warning of tumor susceptibility, unscientific and other problems

Active Publication Date: 2018-11-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the occurrence and development of tumors is a very complicated process, and it is obviously impossible and unscientific to use the changes of a single molecular genetic marker to diagnose the disease
With the existing

Method used

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  • Kit and system for predicting susceptibility of laryngeal squamous cell carcinoma
  • Kit and system for predicting susceptibility of laryngeal squamous cell carcinoma
  • Kit and system for predicting susceptibility of laryngeal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Example 1 A susceptibility prediction system for laryngeal squamous cell carcinoma

[0111] A susceptibility prediction system for laryngeal squamous cell carcinoma, comprising:

[0112] A device for obtaining the number of repetitions of the STR site short tandem sequence of the sample DNA;

[0113] Data processing and judging device, it comprises following module (as figure 1 ):

[0114] The data input module is used to input the age, gender, and repeat times of the short tandem sequence of the subject;

[0115] The database management module is used for the operation management of data storage, modification, deletion, query and printing;

[0116] The data calculation module is used to calculate the result of the discriminant function according to the number of repetitions of the STR site short tandem sequence in the data input module;

[0117] The analysis, discrimination and result output module is used to compare the results of the discriminant function, so as...

Embodiment 2

[0153] Example 2 A susceptibility prediction kit for laryngeal squamous cell carcinoma

[0154] A susceptibility prediction kit for laryngeal squamous cell carcinoma, which comprises the following components: STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, STR-6 primers, PCR Amplification reaction solution, LIZ-500 molecular weight internal standard, deionized formamide, instruction manual.

[0155] The concentrations of the above STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers are all 10 μM, and the primer sequences are shown in the following table:

[0156]

[0157]

[0158] The PCR amplification reaction solution is a mixture of the following reagents: TaqDNA polymerase (5U / μL), Tris-HCl (100mM, pH 8.8 at 25°C), KCl (500mM), ethylphenyl polyethylene glycol (0.8 %(v / v)), MgCl 2 (25mM), dNTP (10mM), deionized water.

[0159] The PCR amplification reaction solution is stored at -20°C; the LIZ-500 ...

Embodiment 3

[0161] Example 3 Using the system of Example 1 and the kit of Example 2 to predict the risk of the subject suffering from laryngeal squamous cell carcinoma

[0162] Subject: Male, 59 years old, visited the Department of Otorhinolaryngology, China-Japan Friendship Hospital of Jilin University. After fully informing the purpose and use of the examination, and on the premise of his willingness, he signed an informed consent and collected 1 mL of anticoagulant blood via peripheral vein. .

[0163] Use the kit of embodiment 2, carry out the following steps according to the method recorded on the kit instruction manual:

[0164] (1) Extract sample DNA: Use a DNA extraction kit to extract blood genomic DNA;

[0165] (2) PCR reaction

[0166] (2-1) Take out STR-1 primer, STR-2 primer, STR-3 primer, STR-4 primer, STR-5 primer, STR-6 primer, PCR amplification reaction solution from the refrigerator, equilibrate to room temperature, each After the components are fully dissolved, quick...

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Abstract

The invention relates to a kit for predicting susceptibility of laryngeal squamous cell carcinoma. The kit comprises the following components: an STR-1 (Short tandem repeats) primer, an STR-2 primer,an STR-3 primer, an STR-4 primer, an STR-5 primer, an STR-6 primer, and also comprises a PCR (Polymerase Chain Reaction) amplification reaction solution, an LIZ-500 molecular weight internal lane standard, and deionized formamide. The kit for predicting susceptibility of laryngeal squamous cell carcinoma provided by the invention can be used for diagnosis and susceptibility prediction of laryngealsquamous cell carcinoma. The invention also provides a system for predicting susceptibility of laryngeal squamous cell carcinoma.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, it relates to a susceptibility prediction kit for laryngeal squamous cell carcinoma and a susceptibility prediction system for laryngeal squamous cell carcinoma. More specifically, the present invention relates to a kit for detecting STRs of genes related to susceptibility to laryngeal squamous cell carcinoma by means of short tandem repeats (Shorttandem repeats, STR for short) locus fragment analysis method, and by combining discriminant analysis statistical methods, In this way, the susceptibility of the subjects to laryngeal squamous cell carcinoma can be early warned. Background technique [0002] Tumor is a disease closely related to genetics. To study the molecular genetic basis of tumors, and then propose tumor-specific genetic markers, it is hoped that it can be used for routine detection, clinical diagnosis, personalized treatment, and disease tracking after recovery. E...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869G06F19/18G06F19/24
CPCC12Q1/6869C12Q1/6886C12Q2600/156C12Q2525/151C12Q2531/113C12Q2537/165
Inventor 王晓峰何金婷杨麒巍郝书弘任明
Owner JILIN UNIV
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