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Method for detecting pathogens with sandwich structure through SERS

A sandwich structure, sandwich technology, applied in measurement devices, nanotechnology for sensing, instruments, etc., can solve the problems of low price of antibiotics, high price of antibodies, low stability, etc., and achieve high detection sensitivity and binding site. Multiple, stable effects

Active Publication Date: 2018-11-23
JINAN UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Antibodies have high specificity, but the price of antibodies is high and their stability is low; aptamers have high stability and high specificity, but the types of aptamers currently screened that can be used to specifically capture bacteria are relatively low. Fewer; antibiotics are low in price, high in stability, and can specifically adsorb bacteria, but antibiotics are small molecules with fewer recognition sites for bacteria

Method used

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  • Method for detecting pathogens with sandwich structure through SERS
  • Method for detecting pathogens with sandwich structure through SERS
  • Method for detecting pathogens with sandwich structure through SERS

Examples

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Embodiment

[0039] (1) Preparation of antimicrobial peptide functionalized magnetic nanoparticles:

[0040] (a) Magnetic Fe3O4 nanoparticles (Fe 3 o 4 NPs) synthesis: the 1.35g FeCl3 ·6H 2 O was added to 40 mL of ethylene glycol and stirred to form a homogeneous solution. Continue to add 1.0g polyethylene glycol (the number average molecular weight is 10000) and 3.6g NaAc 6H 2 O, stir until completely dissolved. The resulting mixture was transferred to a 100 mL autoclave and heated at 200 °C for 8 h. The resulting product was magnetically separated and washed three times with ethanol and deionized water respectively to obtain magnetic Fe 3 o 4 NPs.

[0041] (b) Magnetic Fe 3 o 4 NPs for silanization modification (encapsulation of tetraethyl orthosilicate TEOS): take 3mL 7.5mg / mLFe 3 o 4 NPs solution (dispersed in 1.2wt.% ammonia water), add 200 μL TEOS, sonicate for 90 minutes, continue to add 10 μL TEOS, sonicate for 90 minutes, magnetically separate the obtained product, wash...

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Abstract

The invention belongs to the technical field of biological detection and discloses a method for detecting pathogens with sandwich structure through SERS. The method comprises the following steps thatincubation is performed on antimicrobial peptide functionalized magnetic nanoparticles and bacteria, magnetic nanoparticles which adsorb the bacteria are separated out under the effect of an externalmagnetic field to obtain a magnetic nanoparticle-bacterium compound, then SERS probe co-incubation is performed on a mercaptophenylboric acid modified graphene oxide-silver-coated gold nano-compound to form a sandwich structure formed by antimicrobial peptide functionalized magnetic nanoparticle-bacterium-SERS probe, the sandwich structure is separated out under the effect of the external magneticfield, and detection and identification are performed on different bacteria through SERS.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting pathogenic bacteria by SERS with a sandwich structure. Background technique [0002] Bacteria detection is an important field related to public safety. Traditional methods for bacterial detection include plate counting, polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELSA), etc., which are widely used as sensitive and efficient microbial quantification methods. However, they have shortcomings such as long bacterial culture time, special sample pretreatment, and poor anti-interference ability. Therefore, it is of great significance to develop rapid, sensitive, and specific bacterial detection methods. [0003] Surface-enhanced Raman spectroscopy (SERS) has the advantages of fast, efficient, and high specificity in bacterial detection. Since different substances have their own unique fingerprints, SERS is also a g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y15/00
CPCB82Y15/00G01N21/658
Inventor 周海波江正瑾袁凯松郭鑫杰胡紫微包芷君简敬一
Owner JINAN UNIVERSITY
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