A kind of agncs@apap fluorescent probe and its preparation method and its application in measuring hg(ii)
A fluorescent probe and needle-type technology, applied in the field of fluorescence analysis, can solve the problems of high detection cost, complicated instrument technology, and long time-consuming sample pretreatment process, and achieve the effect of wide linear range and low detection limit
Inactive Publication Date: 2020-10-16
HENAN NORMAL UNIV
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Problems solved by technology
At present, the analytical methods for detecting Hg(II) ions are quite complicated due to the complicated instrument technology, the sample pretreatment process takes a long time, and the detection cost is high.
Method used
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[0012] Preparation of AgNCs@APAP fluorescent probe
[0013] AgNCs@APAP was synthesized by a one-step synthesis method. Take acetaminophen (APAP) (7mL, 50mM) solution in a 50mL jacketed beaker, add AgNO 3 The solution (0.3 mL, 0.1 M) was then added with 2.7 mL of deionized water, and reacted for 10 h at 55° C. with magnetic stirring in the dark. Afterwards, it was filtered with a 0.45 μm hydrophilic PTFE needle filter, and then dialyzed through a 1 kDa dialysis bag, and stored at 4°C in the dark for future use.
[0014] characterize
[0015] figure 1 The UV-Vis absorption spectra of APAP and AgNCs@APAP show that AgNCs@APAP has characteristic absorption bands at 200nm, 245nm and 290nm, which proves that AgNCs@APAP and APAP are not the same substance.
[0016] figure 2 It is the high-resolution transmission electron microscope (a) and the electron diffraction pattern (b) of the selected area of AgNCs@APAP. figure 2 (a) It can be seen that the average particle size of AgN...
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Abstract
The invention belongs to the technical field of fluorescence analysis, and specifically relates to an AgNCs@APAP fluorescent probe and a preparation method thereof, and an application of the AgNCs@APAP fluorescent probe in determination of Hg(II). The AgNCs@APAP fluorescent probe is prepared through the following main steps: weighing a paracetamol (APAP) solution (7 mL, 50 mM), placing the paracetamol solution into a jacketed beaker with a volume of 50 mL, adding an AgNO3 solution (0.3 mL, 0.1 M), then adding water until a mixed solution in the jacketed beaker is 10 mL, and carrying out a light-shielded reaction under magnetic stirring at 55 DEG C for 10 h; and carrying out filtering through a hydrophilic PTFE needle type filter with a pore size of 0.45 [mu]m, and carrying out dialysis through a dialysis bag with a molecular weight of 1 kDa. The fluorescent probe provided by the invention has the advantages of simple synthetic steps, mild reaction conditions, high fluorescence quantumyield and applicability to long-term storage. A system composed of trithiocyanuric acid (TCY), a BR buffer solution (a pH value is equal to 10) and the AgNCs@APAP fluorescent probe is established fordetermination of the Hg(II), and is successfully used for determination of the Hg(II) in a water sample.
Description
technical field [0001] The invention belongs to the technical field of fluorescence analysis, and in particular relates to an AgNCs@APAP fluorescent probe, a preparation method thereof and an application in measuring Hg(II). Background technique [0002] After mercury is discharged into the water, methylmercury is generated under the decomposition of bacteria, enters the organism through the food chain, and accumulates in the organism. In mercury-polluted water bodies, the concentration of methylmercury in fish is tens of thousands of times higher than in unpolluted water. At the same time, methylmercury is easily soluble in lipids, easily absorbed by the human body, has slow metabolism, high cumulative toxicity, and is not easy to decompose. It is a highly toxic nerve agent that accumulates in the brain and damages the kidneys and capillaries. effect. Therefore, proposing a fast, simple, convenient and sensitive method for detecting Hg(II) is of great significance to biol...
Claims
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IPC IPC(8): C07C233/25C09K11/06G01N21/64
CPCC07C233/25C09K11/06C09K2211/188G01N21/6428
Inventor 王治科张柏爽朱韵怡王全坤叶存玲范顺利
Owner HENAN NORMAL UNIV