Mixed storage method for soybeans and sugar grass for feed
A technology of sweet sorghum and soybean, which is applied in the field of silage preparation by mixed storage of forage soybean and sweet sorghum, which can solve the problems of difficulty in rapid formation of dominant flora of lactic acid bacteria, loss of nutrients and energy, prolongation of pre-fermentation period, etc. Sustained development, reduced nutrient loss, good practical value
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Embodiment 1
[0039] Example 1, Isolation, identification and performance measurement of Lactobacillus plantarum (Lactobacillus plantarum) WQ-01
[0040] 1. Isolation of strain WQ-01
[0041] A total of 105 strains were isolated from the surface of forage soybean, whole corn and sweet sorghum plants, and cultured under anaerobic conditions at 30°C for 2 days on MRS solid medium. Pick a single colony, culture each streak twice, then inoculate it on a nutrient liquid medium, and store it at -80°C for later use.
[0042] 2. Screening and identification of strain WQ-01
[0043] 1. Identification of physiological and biochemical characteristics
[0044] The invention identifies the physiological and biochemical characteristics of the isolated bacterial strains including: temperature, acid resistance and the like.
[0045] Temperature resistance test method: Inoculate the isolated strains into lactic acid bacteria liquid culture medium and place them in constant temperature refrigerators at 5°...
Embodiment 2
[0067] Embodiment 2, forage soybean and sweet sorghum mixed storage prepare silage
test approach I
[0069] The whole soybean plant for feed (bulging grain stage, water content 65%) and the whole sweet sorghum plant (milk ripening stage, water content 75%) are harvested on the same day. Before silage, forage soybeans and sweet sorghum were cut into 2cm lengths, mixed evenly according to the weight ratio of forage soybeans and sweet sorghum 1:9, and each bag weighed 20kg, while spraying lactic acid bacteria (Lactobacillus plantarum) WQ-01 bacterial powder (bacteria powder Activity is 10 11 cfu / g), the amount added is 10 per gram of fresh weight 6 cfu (cfu, colony forming unit). Then the silage was manually compacted, vacuum-sealed with polyethylene bags, 5 replicates per treatment group, protected from light and rodents, and stored at room temperature (15-30°C). After 60 days, the bag was opened to detect its nutritional quality and fermentation quality.
[0070] The results showed that the fermentation quality of 10% whole-plant soybean + 90% sweet sorghum mixed silage was...
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