Use of spirulina protease hydrolyzate and preparation method thereof
A technology of proteolysis and spirulina, which is applied in the field of medicine, can solve problems such as anti-aging neurotoxicity protein aggregation and alleviating neurotoxicity of spirulina hydrolyzate, achieve significant neuroprotective effect, improve the quality of life in old age, and biological highly active effect
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Embodiment 1
[0036] Embodiment 1 A kind of spirulina protein hydrolyzate (SPPH)
[0037] Made with the following steps:
[0038]S1. Wall breaking: use Tris-HCl buffer to prepare Spirulina platensis powder into a suspension with a weight and volume percentage of 10%, adjust the pH to 7.4, soak for 1 day, freeze at -20°C for 12 hours, and then place at 4 Thaw at ℃, repeat freezing and thawing 3 times, and centrifuge at 5000rpm for 30min to obtain the supernatant;
[0039] S2. Protein separation: take the supernatant obtained in step S1, slowly add NH 4 SO 4 Until its saturation is 50%, keep stirring, place at 4°C overnight, centrifuge at 5000rpm for 30min, take the precipitate and redissolve it in first-grade water, place it in a 3.5KDa dialysis zone, dialyze it with flowing water for 3d, and then use Dialyze 3 times with deionized water, change the deionized water every 12 hours, take the protein solution after dialysis and freeze-dry to obtain the protein extract of spirulina;
[004...
Embodiment 2
[0041] Embodiment 2 A kind of spirulina protein hydrolyzate (SPPH)
[0042] Made with the following steps:
[0043] S1. Wall breaking: use Tris-HCl buffer to prepare Spirulina platensis powder into a suspension with a weight and volume percentage of 12%, adjust the pH to 7.5, soak for 2 days, freeze at -20°C for 24 hours, and then place it at 4 Thaw at ℃, and after repeated freezing and thawing 4 times, centrifuge at 6000rpm for 10min to obtain the supernatant;
[0044] S2. Protein separation: take the supernatant obtained in step S1, slowly add NH 4 SO 4 Until its saturation is 50%, keep stirring, place at 4°C overnight, centrifuge at 6000rpm for 10min, take the precipitate and redissolve it in first-grade water, place it in a 3.5KDa dialysis zone, dialyze it with running water for 3 days, and then use Dialyze 4 times with deionized water, change deionized water every 12 hours, take the protein solution after dialysis and freeze-dry to obtain spirulina protein extract; ...
Embodiment 3
[0046] Embodiment 3 A kind of spirulina protein hydrolyzate (SPPH)
[0047] Made with the following steps:
[0048] S1. Wall breaking: use Tris-HCl buffer solution to prepare Spirulina platensis powder into a suspension with a weight volume percentage of 8%, adjust the pH to 7.3, soak for 2 days, freeze at -20°C for 18h, and then place it at 25°C. Thaw at ℃, after repeated freezing and thawing 5 times, centrifuge at 8000rpm for 10min to obtain the supernatant;
[0049] S2. Protein separation: take the supernatant obtained in step S1, slowly add NH 4 SO 4 Until its saturation is 50%, keep stirring, place at 4°C overnight, centrifuge at 8000rpm for 10min, take the precipitate and redissolve it in first-grade water, place it in a 3.5KDa dialysis zone, dialyze it with flowing water for 3 days, and then use Dialyze 5 times with deionized water, change deionized water every 12 hours, take the protein solution after dialysis and freeze-dry to obtain spirulina protein extract;
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