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Serum small dense low-density lipoprotein cholesterol detection kit

A low-density lipoprotein and detection kit technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., to achieve the effects of good stability, guaranteed accuracy and simple use

Inactive Publication Date: 2018-12-07
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0017] Evaluation: This method has the advantages of rapid detection and analysis of samples, high accuracy, and simple operation, but requires special and expensive experimental equipment such as nuclear magnetic resonance, so NMR spectroscopy cannot be widely used in laboratories

Method used

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  • Serum small dense low-density lipoprotein cholesterol detection kit
  • Serum small dense low-density lipoprotein cholesterol detection kit
  • Serum small dense low-density lipoprotein cholesterol detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]

[0041] R2:

[0042]

[0043] (2) The usage method of the reagent of this embodiment:

[0044] The serum small and dense low-density lipoprotein cholesterol (peroxidase method) detection reagent described in this embodiment adopts an automatic biochemical analyzer during use, such as Hitachi 7180 automatic analyzer, etc., and utilizes the endpoint method for determination. Set the ratio of sample and reagent R1, R2 to 3:300:100, the detection wavelength is 600nm, place distilled water, standard and sample in the corresponding position of the sample plate, the operation is shown in Table 1:

[0045] Table 1 Example 1 reagent detection method

[0046]

[0047] Serum small dense low-density lipoprotein cholesterol content (mmol / L)=(sample ΔA) / (standard ΔA)×standard concentration.

Embodiment 2

[0049] Interference test: take fresh mixed serum, divide it into 2 equal parts, then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then respectively use the reagent obtained in Example 1 to compare and measure the content of serum small and dense low-density lipoprotein cholesterol simultaneously with the common and recognized serum small and dense low-density lipoprotein cholesterol reagents in the market. The measurement results of each group are shown in Table 2. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measuring mean value of control samples × 100%.

[0050] Table 2 embodiment reagent anti-interference performance comparison

[0051]

[0052]As can be seen from Table 2, the reagent of embodiment 1 has no effect on the test results when bilirubin≤300 μmol / L, creatinine≤200 μmol / L, hemogl...

Embodiment 3

[0054] Correlation experiment: Utilize the formula preparation reagent of embodiment 1, carry out control detection with the small and dense low-density lipoprotein cholesterol (peroxidase method) kit of a certain company approved by the State Food and Drug Administration common in the market, detect simultaneously Twenty clinical serum samples were collected, and the test results are shown in Table 3. And obtained the correlation curve of the two reagents (such as figure 1 As shown), the test results show that the correlation coefficient of the two kits is 0.999, which shows that there is a great correlation between the two.

[0055] Table 3 embodiment 1 reagent and control group kit comparative detection result

[0056]

[0057] The calibrators and quality controls used in the test were:

[0058] Calibrator: the content of serum small dense low-density lipoprotein cholesterol is 1.43mmol / L.

[0059] Quality Control: The target value of serum small dense LDL cholesterol...

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Abstract

The invention relates to the technical field of serum small dense low-density lipoprotein cholesterol (peroxidase method) detection, and particularly relates to a serum small dense low-density lipoprotein cholesterol detection reagent. The reagent R1 contains MOPS buffer solution having pH of 7.0, cholesterol esterase, cholesterol oxidase, sphingomyelinase, catalase, polyethylene glycol 3-benzyloxy-phenyl ether, JJ70L, TOOS, 4-tolylboronic acid (4-FPBA), D-trehalose and lithium chloride; and the reagent R2 contains MOPS buffer solution having pH of 7.0, peroxidase, TritonX-100, 4-AA and lithium chloride. The reagents are applicable to various full-automatic biochemical analyzers, and have excellent accuracy, interference resistance and excellent thermal stability.

Description

technical field [0001] The invention relates to the technical field of serum small and dense low-density lipoprotein cholesterol detection, in particular to a serum small and dense low-density lipoprotein cholesterol detection reagent suitable for an automatic biochemical analyzer. Background technique [0002] 1. Background [0003] Small, dense low-density lipoprotein cholesterol (sd LDL) is one of the subfractions of low-density lipoprotein cholesterol (LDL). LDL is divided into three components, including: LDL, with a molecular radius of 30-80nm; L LDL, with a molecular radius of 25.5-28.0nm; sdLDL: a molecular radius of 22.0-25.5nm. sd LDL is high-density LDL. 2. Clinical significance [0004] In the occurrence and development of atherosclerosis (AS), the deposition of lipoproteins on the arterial wall is a series of processes. It first resides in a certain part of the arterial wall, and then is taken up by mononuclear macrophages or smooth muscle cells. The extrace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/30C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/30C12Q1/44C12Q1/60
Inventor 赵民胡晓飞罗维晓王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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