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A kind of preparation method of L-glutamine

A glutamine and crystallization technology, applied in the field of amino acid production, can solve the problems of large acid and alkali, high production cost, environmental pollution and the like, and achieve the effects of improving yield, reducing pollution and reducing energy consumption

Active Publication Date: 2021-10-01
MEIHUA BIOTECH LANGFANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of anion and cation exchange resins needs to be regenerated with alkali and acid, and rinsed with clean water before they can be put into use again. This process consumes a lot of acid and alkali, and produces a lot of sewage, resulting in serious environmental pollution and high production costs.

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  • A kind of preparation method of L-glutamine
  • A kind of preparation method of L-glutamine
  • A kind of preparation method of L-glutamine

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[0024] The invention discloses a preparation method of L-glutamine, and those skilled in the art can refer to the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0025] The invention provides a method for extracting glutamine from fermented liquid. The extraction process of twice crystallization and one decolorization adopted in the invention has the advantages of ...

Embodiment 1

[0041] Embodiment 1 extracts L-glutamine from fermented liquid

[0042] Take 30L of fermentation broth, the fermentation broth produces 85g / L of acid, use 150kda ceramic membrane microfiltration, the temperature is 42°C, the transmembrane pressure difference is 2.0bar, the concentration factor is 10 times, and the filtrate is concentrated and crystallized to glutamine The mass percentage of L-glutamine is 25%; cooled to 5° C., pre-crystallized for 10 hours, and the crude product of L-glutamine is separated and obtained, the crude product is 3250 g, and the moisture content of the crude product is 32%.

[0043] Add deionized water, make 43L of L-glutamine 5.0% crude product solution at 40°C, add 215g medicinal carbon for decolorization and stir for 30min to obtain decolorized clear night; The mass percentage of aminoamide is 30%; the temperature is lowered by 10°C every hour to 8°C for separation, and the wet product is dried to obtain 1700g of L-glutamine finished product.

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Embodiment 2

[0045] Embodiment 2 extracts L-glutamine from fermented liquid

[0046] Take 20L of fermentation broth, acid production of fermentation broth is 83g / L, using 150kda ceramic membrane microfiltration, the temperature is 35°C, the transmembrane pressure difference is 3.6bar, and the concentration factor is 8 times, and the filtrate is concentrated and crystallized, and concentrated to glutamine The mass percentage of L-glutamine was 24%; cooled to 5° C., pre-crystallized for 10 hours, and the crude product of L-glutamine was separated, 2120 g of the crude product, and 33% of the moisture content of the crude product.

[0047] Add deionized water, make 30.6L of L-glutamine 4.5% crude product solution at 35°C, add 125g of medical carbon for decolorization and stir for 30min to obtain decolorized clear night, the decolorization solution is concentrated and crystallized, the evaporation temperature is ≦40°C, and concentrated to The mass percentage of glutamine is 28%; the temperature...

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Abstract

The invention relates to the technical field of amino acid production, in particular to a method for extracting L-glutamine from a fermentation broth. The invention provides a method for preparing L-glutamine. The L-glutamine fermentation broth is obtained through microfiltration, crystallization and decolorization to obtain L-glutamine; the ion exchange step is not included. The preparation method provided by the invention completely removes the step of ion exchange, greatly increases the yield, saves acid, alkali and water, greatly reduces waste water and waste liquid, reduces pollution, reduces energy consumption, and saves costs . The by-product retained liquid and crude mother liquor are sent to Lvnong workshop to make fertilizers, and the invention realizes zero discharge of the glutamine extraction process.

Description

technical field [0001] The invention relates to the technical field of amino acid production, in particular to a preparation method of L-glutamine. Background technique [0002] Glutamine, scientific name 2-amino-5-carboxypentanamide, L-glutamine is an encoded amino acid in protein synthesis, a non-essential amino acid for mammals, and it is easy to decompose into glutamine under partial acidity, partial alkalinity and high temperature The acid may be cyclized into pyrrolidone dicarboxylic acid, its chemical molecular formula is C5H10O3N2, its molecular weight is 146.15, and its decomposition temperature is 184-185°C. [0003] L-glutamine has various physiological effects on humans and animals. Glutamine has pharmacological effects such as promoting the repair of mucosal epithelial tissue, helping to eliminate ulcer lesions, promoting brain metabolism, and improving brain function. Studies have shown that glutamine can protect the intestinal mucosal barrier function and pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/22C07C233/05
CPCC07C231/22C07C233/05
Inventor 吴涛白晨龙王海雷龚华李岩赵津津乔石磊何阳
Owner MEIHUA BIOTECH LANGFANG CO LTD
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