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Preparation method of anti-breast-cancer active component in macleaya cordata

A technology of broad-based separation and separation method, which is applied in the fields of antineoplastic drugs, organic chemistry, drug combination, etc., can solve problems such as lack of reference value, and achieve the effects of simple operation, high purity of separation products, and high extraction efficiency

Active Publication Date: 2018-12-14
SHANDONG UNIV
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  • Claims
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Problems solved by technology

Different Chinese medicine extracts contain different components, as well as the types and quantities of alkaloids. Therefore, in the face of different Chinese medicines and different extracts, the types of solvents in the solvent system and the dosage ratio between solvents are not available for reference. valuable

Method used

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  • Preparation method of anti-breast-cancer active component in macleaya cordata
  • Preparation method of anti-breast-cancer active component in macleaya cordata
  • Preparation method of anti-breast-cancer active component in macleaya cordata

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Embodiment 1

[0034] 1. Sample Extraction

[0035] Dissolve the crude Boluohui alkaloid sample in an aqueous solution with a pH value of 2 (adjusted with HCl), filter through a medium-speed filter paper of 30–50 μm, and adjust the pH value of the filtrate to 9 with 10% dilute ammonia water. After the alkaloid is fully precipitated, Use a centrifuge to centrifuge at 3000 rpm, remove the upper layer solution, and the lower layer of sediment, that is, Deborah, will fall back to the total alkaloid sample. 2. Application of pH zone countercurrent chromatography to separate the total sample of Boluohui alkaloids

[0036]Prepare the two-phase solvent system n-hexane / ethyl acetate / isopropanol / water (1:3:1.5:4.5, v / v) according to the above-mentioned solvent ratio, place it in a separatory funnel, shake well and let stand Separate the upper and lower phases after equilibrating for a period of time, add 10mmol triethylamine to the upper phase as a retained base, add 10mmol trifluoroacetic acid to th...

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Abstract

The invention discloses a method for separating alkaloid compounds in macleaya cordata through pH-zone-refining counter-current chromatography. An environment-friendly n-hexane / ethyl acetate / isopropylalcohol / water solvent system is adopted for separation, and the volume ratio of al reagents in the solvent system is 1:3:1.5:4.5. Sanguinarine and chelerythrine with the initial purity of 98% or above can be efficiently separated from the macleaya cordata with the method. Besides, anti-breast-cancer effect evaluation tests prove that the sanguinarine and the chelerythrine obtained with the extraction method have anti-breast-cancer activity and have a synergistic effect, and a new thought is provided for research and development of anti-breast-cancer drugs.

Description

technical field [0001] The invention relates to a method for separating and purifying active ingredients of traditional Chinese medicines, in particular to a method for separating alkaloid compounds (sanguinarine and chelerythrine) in Boluohui by using pH zone countercurrent chromatography. Background technique [0002] Macleaya cordata (Willd.) R.Br. is the above-ground part of the poppy family Macleaya cordata (Willd.) R.Br., mainly distributed in China and Southeast Asia, and mainly distributed in Hunan, Anhui, Jiangsu, Zhejiang and other provinces in China. Boluohui is rich in alkaloids. Modern pharmacology shows that Boluohui has the effects of promoting animal growth, antibacterial, anti-inflammatory, and anti-tumor effects. The products developed based on Boluohui mainly include Meiyou Zhuang, Boluohui Injection, Boluohui Swelling and Itching Tincture, etc. The traditional separation methods of alkaloid monomer components include silica gel column chromatography, pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/153C07D491/056A61P35/00
CPCA61P35/00C07D491/056C07D491/153
Inventor 孙蓉王岱杰李晓宇刘闰平李晓骄阳黄娜娜
Owner SHANDONG UNIV