Biomarker long-chain non-coding RNA LINC01728 and kit for predicting prognosis of colon cancer

A biomarker and long-chain non-coding technology, which is applied in the field of biomarker long-chain non-coding RNALINC01728 and kits, can solve problems that have not been reported, improve survival rate, improve postoperative quality of life, and have far-reaching clinical significance Effect

Inactive Publication Date: 2018-12-14
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether LINC01728 can be used as a new tumor marker to predict the prognosis of patients with colon cancer has not been reported

Method used

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  • Biomarker long-chain non-coding RNA LINC01728 and kit for predicting prognosis of colon cancer
  • Biomarker long-chain non-coding RNA LINC01728 and kit for predicting prognosis of colon cancer
  • Biomarker long-chain non-coding RNA LINC01728 and kit for predicting prognosis of colon cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The biomarker used to predict the prognosis of colon cancer is long-chain non-coding RNA LINC01728, and the nucleic acid sequence of the long-chain non-coding RNA LINC01728 is shown in SEQ NO:1.

[0019] The preparation for predicting the prognosis of colon cancer is a real-time fluorescent quantitative PCR detection kit, a real-time fluorescent quantitative PCR detection kit, including specific primers for detecting the expression of the target gene LINC01728, the primer sequences are SEQ NO:2 and SEQNO:3, real-time fluorescent The quantitative PCR detection kit also includes specific primers for the expression of the internal reference gene TUBA1A, and the primer sequences are SEQ NO:4 and SEQ NO:5.

[0020] Preparation of reagents for detecting the expression of LINC01728 is used to prepare a kit for the prognosis of patients with colon cancer (for 50 reactions), and the required reagents include: SYBR Green qPCR Mix 500 μL, 3 μM target gene LINC01728-specific primer ...

Embodiment 2

[0024] Detection of LINC01728 in Tissue Samples

[0025] 1. Collect the colon cancer tumor tissue or normal control tissue to be tested, wash it with normal saline, put it into a cryopreservation tube filled with a solvent that inhibits RNA degradation, and put it in a -80°C refrigerator for later use.

[0026] 2. Extraction of RNA from tissues

[0027] (1) Add liquid nitrogen to the mortar first, then cut the tissue into small pieces and grind it into powder in liquid nitrogen, take 100mg of tissue powder with a liquid nitrogen pre-cooled spoon and add it to the EP tube filled with 1ml of Trizol solution middle. The total volume of tissue powder should not exceed 10% of the volume of Trizol used, and it should be thoroughly mixed evenly.

[0028] (2) Leave at room temperature for 5 minutes, then add 200 μL of chloroform, tightly cap the EP tube and shake vigorously for 0.5 minutes. Centrifuge at 12,000 rpm for 10 minutes.

[0029] (3) Take the upper aqueous phase into a n...

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Abstract

The invention provides a biomarker long-chain non-coding RNA LINC01728 and a kit for predicting the prognosis of colon cancer, which are used for predicting the prognosis survival time of a patient with colon cancer. A preparation for predicting the prognosis of colon cancer is a real-time fluorescent quantitative PCR detection kit, and the kit contains a specific primer h used for detecting the expression of a target gene LINC01728 and the specific primer used for detecting the expression of a reference gene TUBA1A, and further contains real-time fluorescent quantitative SYBR dye and RNase-free water, wherein the volume ratio of the real-time fluorescent quantitative SYBR dye to the target gene LINC01728-specific prime to the reference gene TUBA1A-specific primer to the RNase-free water in the kit is 10 to 1 to 1 to 6. This method provides a strong technical support for predicting the prognosis survival time of a patient with colon cancer, facilitates the improvements on the postoperative life quality of the patients with colon cancer and the development of postoperative treatment plans, improves the survival rate, and has a far-reaching clinical significance.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to long-chain non-coding RNA LINC01728, a biomarker for predicting the prognosis of colon cancer, and a kit. Background technique [0002] Colon cancer is a common malignant tumor originating from the intestinal mucosa. However, with the change of living environment and China's gradually westernized diet and lifestyle, the incidence of colon cancer has increased year by year. Colon cancer has developed into the third malignant tumor in my country . Colon cancer mostly occurs in middle-aged and elderly people, and the peak age of onset is about 50 years old. However, with the change of social environment, the incidence pattern of colon cancer is gradually changing, and the incidence rate of young people is getting higher and higher. Young colon cancer is different from elderly colon cancer and has unique molecular pathological features. It has the characteristics of a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/178
Inventor 田田
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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