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Preparation method of acellular bovine pericardium matrix capable of slowly releasing bioactive factors

A technology of biologically active factors and acellular matrix, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as poor remodeling effect and difficult endogenous growth of cells, so as to promote host stem cell adhesion and rejuvenation in the body. The effect of good plasticity and slow release of bioactive factors

Inactive Publication Date: 2018-12-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although ionic detergents can achieve a good decellularization effect, the residual toxicity, easy removal of soluble bioactive factors, and dense matrix material structure make it difficult for cells to grow endogenously, and the remodeling effect in the host is not good. Bottlenecks that limit its processing of clinical tissue specimens

Method used

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Embodiment Construction

[0018] In order to make the technical means, creative features, goals and effects of the present invention easy to understand, the following examples will specifically describe the preparation method of the decellularized bovine pericardial matrix capable of slow-releasing bioactive factors of the present invention.

[0019] The preparation method of the decellularized bovine pericardium matrix capable of slow-releasing bioactive factors mainly includes the following steps:

[0020] Step 1, under vacuum conditions, place the bovine pericardium in a non-ionic detergent buffer and freeze for 2-6 hours. The non-ionic detergent buffer includes: Tris buffer, non-ionic detergent and deoxycholesterol Sodium acid. The nonionic detergent was polyethylene glycol octylphenyl ether (Triton X-100). The concentration range of Tris buffer is 8~12mmol / L, the pH value is 7.4~7.8, the volume fraction of Triton X-100 in Tris buffer is in the range of 0.8%~1.5%W / V, sodium deoxycholate is in Tris...

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Abstract

The invention provides a preparation method of an acellular bovine pericardium matrix capable of slowly releasing bioactive factors, and the preparation method comprises the following steps: Step 1, placing bovine pericardium in a non-ionic detergent buffer solution for freezing under vacuum for 2-6h; Step 2, dissolving the frozen bovine pericardium in a water bath; Step 3, repeating the step 1 and the step 2 for 1 to 10 times; Step 4, placing the frozen-thawed bovine pericardium in the non-ionic detergent buffer solution for shocking at 30-50 DEG C for 28-96h; Step 5, cleaning the bovine pericardium with sterile deionized water for 12-48h; Step 6, cleaning by shocking the bovine pericardium with a phosphate solution for 48-96h to obtain a acellular bovine pericardium matrix; and Step 7, immersing the acellular bovine pericardium matrix in a bioactive factor agarose material for 20 min to 2 h to obtain the acellular bovine pericardium matrix capable of slowly releasing the bioactive factors.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a preparation method of decellularized bovine pericardial matrix capable of slow-releasing bioactive factors. Background technique [0002] Tissue engineering is to inoculate living cells on the tissue matrix to produce a biological material with good compatibility with the recipient tissue, no immunogenicity, no need for anticoagulation, and strong durability. Among them, natural matrix materials are one of the important contents of tissue engineering research. An ideal natural matrix material should have the following advantages: (1) good tissue compatibility and no biotoxicity; (2) have a three-dimensional structure, and the material should be porous and sponge-like, which is conducive to cell adhesion and infiltration, and the absorption of nutrients. (3) rich in biologically active factors, providing a good microenvironment for the growth of host cells; (4) having good mec...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/50A61L27/54A61L27/36
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/507A61L27/54A61L27/56A61L2300/414A61L2300/602A61L2430/20A61L2430/22A61L2430/40
Inventor 刘晓红吴昊徐志云夏翠萍
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY