A preparation method of in-situ immobilized laccase ultrafiltration membrane, a treatment method of phenol-containing wastewater

A technology of in situ fixation and treatment method, applied in biological water/sewage treatment, water/sludge/sewage treatment, ultrafiltration, etc., can solve the problems of unrecoverable free laccase, limited application, difficult to achieve reuse, etc.

Active Publication Date: 2021-05-07
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since free laccase is very sensitive to environmental conditions, and free laccase cannot be recycled after participating in the reaction, it is difficult to realize reuse, which limits its practical application.

Method used

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  • A preparation method of in-situ immobilized laccase ultrafiltration membrane, a treatment method of phenol-containing wastewater
  • A preparation method of in-situ immobilized laccase ultrafiltration membrane, a treatment method of phenol-containing wastewater
  • A preparation method of in-situ immobilized laccase ultrafiltration membrane, a treatment method of phenol-containing wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for preparing an in-situ immobilized laccase ultrafiltration membrane, comprising the following steps:

[0037] 1) Take 5 mg of nano-activated carbon Nac with a diameter of 30 nm and 3 mL of 1052 U / mL laccase solution at 25 ° C for 12 h, centrifuge, wash the precipitate with pH = 7 citric acid-disodium hydrogen phosphate buffer solution, and detect in the supernatant If there is no enzyme activity, the immobilized laccase product Lac / Nac is obtained, and the catalytic ability of the immobilized laccase obtained under this condition is 168.4U / g carrier.

[0038] 2) Weigh 1.5g of TB, 8.5g of NMP and 0.8g of Lac / Nac into a conical flask, add a rotor, plug the cap tightly, wrap the mouth of the bottle with plastic wrap and stir it magnetically for 24 hours. Put it into a vacuum drying oven for degassing at 60°C for 1 hour to obtain a casting solution.

[0039] 3) Scrape the prepared film-casting solution in a film-scraping machine under the following conditions: t...

Embodiment 2

[0042] Determination of thermal stability: use citric acid-disodium hydrogen phosphate buffer solution (pH=4) to prepare a solution with guaiacol (concentration: 1.0mmol / L) as the substrate, take 8 parts of 4 mL each, and add 500 mg for implementation The Lac / Nac@TB prepared in Example 1 was stirred and reacted in a water bath at 10, 20, 30, 40, 50, 60, 70, and 80°C for 30 minutes respectively, centrifuged to take the supernatant, and measured its absorbance (OD 465 ) value, measure the relative enzymatic activity of Lac / Nac@TB, the result is as follows Figure 6 shown. It can be seen that Lac / Nac@TB has good thermal stability. When the temperature is between 20°C and 60°C, the relative enzyme activity of Lac / Nac@TB still maintains more than 64.7%, which shows that under high temperature conditions, laccase molecules are It is fixed in the Nac@TB channel in situ, and there is a certain temperature difference with the external environment to avoid the inactivation of enzyme mo...

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PUM

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Abstract

The invention provides a preparation method of an in-situ immobilized laccase ultrafiltration membrane and a treatment method of phenol-containing wastewater. Compared with the prior art, the present invention introduces nano-carbon powder of immobilized laccase into an organic matrix ( Nac@TB) prepared an organic-inorganic hybrid ultrafiltration membrane (Lac / Nac@TB) with immobilized laccase. Scanning electron microscopy and fluorescence optical microscopy showed that Lac / Nac@TB had sponge-like nanoscale pores inside, and laccase was successfully synthesized. In situ embedding and immobilization inside Lac / Nac@TB, the immobilized laccase has good pH stability and thermal stability, and the degradation rate of phenolic pollutants reaches 78.4-81.5%.

Description

technical field [0001] The invention belongs to the field of wastewater treatment, and in particular relates to a preparation method of an in-situ immobilized laccase ultrafiltration membrane and a treatment method of phenol-containing wastewater. Background technique [0002] Phenolic compounds are one of the main pollutants, and they are also regarded as priority pollutants. Even at low concentrations, phenolic pollutants in drinking water will cause harm to human health. [0003] Laccase is a copper-containing polyphenol oxidase. Studies have shown that it has a strong ability to catalyze the oxidation of dyes and phenols, and has shown great potential in wastewater treatment. Because free laccase is very sensitive to environmental conditions, and free laccase cannot be recovered after participating in the reaction, it is difficult to realize reuse, which limits its practical application. Therefore, immobilization technology is often used to improve the stability and reu...

Claims

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Application Information

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IPC IPC(8): B01D67/00B01D69/14B01D69/02B01D61/14C02F3/34C02F101/34
CPCB01D61/145B01D67/0079B01D69/02B01D69/144B01D69/148B01D2325/026B01D2325/22B01D2325/30C02F3/342C02F2101/345
Inventor 唐海张晨李强张昊楠秦宝雨徐建平
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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