GFP-2 nano oligopeptide collagen and a preparing method and application thereof
A technology of GFP-2 and culture medium, which is applied in the preparation of small peptide adjuvants derived from marine bacillus GFP-2, and in the application field of immune efficacy, which can solve the problems of low immunogenicity and inability to achieve immune body efficacy, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0025] First of all, the present invention provides a method for preparing GFP-2 small peptide, which includes the following steps:
[0026] (1) Prepare brown sugar medium and sterilize it;
[0027] (2) Inoculate GFP-2 bacteria, inoculate glycerol bacteria with LB medium, and culture on a shaker;
[0028] (3) Transfer to brown sugar medium, culture on a shaker, then centrifuge, collect the supernatant, and discard the bacteria;
[0029] (4) Salting out to make the protein and small peptides precipitate out. After the salting out, centrifuge again to remove the supernatant and collect the crude protein;
[0030] (5) Resuspend the crude protein with tris-HCl, pass the resuspended crude protein over the membrane, and dialyzed with tris-HCl;
[0031] (6) Perform ultrafiltration with an ultrafiltration tube, collect small peptides with a molecular weight less than 10KD, cool the obtained small peptides, and freeze-dry to obtain GFP-2 small peptides.
[0032] In the context of the present inven...
Example Embodiment
[0044] Example 1
[0045] Preparation of GFP-2 small peptide
[0046] (1) Prepare 1 L brown sugar medium and sterilize at 110°C for 15 min;
[0047] (2) Inoculate GFP-2 bacteria, 5mL LB medium per tube, and inoculate glycerol bacteria and medium in a ratio of 1:100. A total of 2 tubes of GFP-2 bacterial solution were taken and cultured on a shaker for 12 hours;
[0048] (3) Transfer to 1L brown sugar medium, culture on a shaker for 12 hours, centrifuge at 4000 rpm for 1 hour, collect the supernatant, and discard the bacteria;
[0049] (4) Prepare saturated ammonium sulfate, salt out the supernatant and saturated ammonium sulfate in a ratio of 4:1 to make the protein and small peptides precipitate out, centrifuge again after the salting out, centrifuge at 12000 rpm for 20 minutes, remove the supernatant, and collect the crude protein
[0050] (5) Resuspend the crude protein with tris-HCL, and pass the resuspended crude protein through a 0.45mm filter membrane, dialyzed with tris-HCL for ...
Example Embodiment
[0052] Example 2
[0053] Study on GFP-2 small peptide to improve antibody titer
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap