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SNP molecular marker related to body size traits of Macrobrachium rosenbergii and application thereof

A technique of Macrobrachium rosenbergii and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., to achieve the effects of accelerating genetic progress, saving production costs, large economic application value and scientific research value

Pending Publication Date: 2018-12-18
FOSHAN UNIVERSITY
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Problems solved by technology

At present, the breeding area of ​​Macrobrachium rosenbergii in China is as high as more than 600,000 mu, but the number of fine species of Macrobrachium rosenbergii is far from meeting its huge industrial scale and huge seed demand. Therefore, the early breeding of Macrobrachium rosenbergii is very urgent and significant

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  • SNP molecular marker related to body size traits of Macrobrachium rosenbergii and application thereof
  • SNP molecular marker related to body size traits of Macrobrachium rosenbergii and application thereof
  • SNP molecular marker related to body size traits of Macrobrachium rosenbergii and application thereof

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Embodiment

[0025] 1. Detection of SNP molecular markers

[0026] 1. Designing primers: designing a pair of primers for specifically amplifying the gene fragment containing the T574C site of the Macrobrachium rosenbergii ND1 gene.

[0027] Utilize the software Primer 5.0, use the ND1 gene (GenBank: NC_006880.1) of Macrobrachium rosenbergii in GenBank as the specificity amplification primer of reference design, the primer sequence that amplifies is

[0028] Upstream F: 5'-GATAAAGTAGGCTATGCTGG-3' (SEQ ID №1), 20bp;

[0029] Downstream R: 3'-CCTCCTCTGTACTCGGTATT-5' (SEQ ID No. 2), 20 bp.

[0030] The optimum temperature is 48.8°C, the product length is 542bp, and it was synthesized by Guangzhou Sangon Bioengineering Co., Ltd.

[0031] 2. Extraction of Macrobrachium rosenbergii Genomic DNA

[0032] Using OMEGAD whole blood kit, the specific steps are as follows:

[0033] (1) Take 150 μL blood sample for each sample, add Elution Buffer, and vortex for 10 seconds;

[0034] (2) Add 500 μL o...

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Abstract

The invention discloses a SNP molecular marker related to body size traits of Macrobrachium rosenbergii and application thereof in the technical field of molecular marker breeding. The SNP molecular marker corresponds to the T574C site of the ND1 gene of Macrobrachium rosenbergii, wherein the base is T or C. The SNP molecular marker of the invention is related to body size traits (carapace height,uromere height and telson height) of Macrobrachium rosenbergii, and is a new molecular marker. By determining the genotype of the SNP site of Macrobrachium rosenbergii to be tested, early breeding ofMacrobrachium rosenbergii can be carried out according to the body size traits (carapace height, uromere height and telson height) of Macrobrachium rosenbergii. Thereby, production costs can be effectively saved and genetic progress can be accelerated, better serving the breeding of Macrobrachium rosenbergii. The SNP molecular marker can satisfy the huge market demand and has great economic application value and scientific value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker breeding, in particular to a SNP molecular marker related to the body size trait of Macrobrachium rosenbergii and its application. Background technique [0002] Marker Assisted Selection (MAS) uses the characteristics of molecular markers closely linked with genes that determine target traits, and screens important economic traits (such as weight, growth rate, stress resistance, etc.) in the process of animal and plant breeding. Related molecular markers, and construct a breeding population with related molecular markers in the breeding process to achieve the purpose of selecting target traits. It has the characteristics of strong targeting and high accuracy, and can greatly improve Breeding efficiency, shorten the breeding period. [0003] Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms, SNP) is a base variation produced by a single nucleotide in the genome, which has t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 冯艺陈文强李镇养李浩杰袁启志张志浩陈文坚杨虹刘咸陈建酬
Owner FOSHAN UNIVERSITY