Shikimic acid extraction method

An extraction method and a technique for shikimic acid, applied in the field of extraction of shikimic acid, can solve problems such as low utilization of raw materials, few sources of shikimic acid, etc., and achieve the effects of high raw material utilization rate, reduction of raw material usage, and simple process

Inactive Publication Date: 2018-12-21
南宁市黄陈生猪养殖场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the defects of less sources of shikimic acid and low utilization of raw materials in the prior art, the present invention provides a method for extracting shikimic acid, which can extract shikimic acid from natural substances such as star anise, cedar, ginkgo leaves, masson pine needles, sweetgum leaves, lemon eucalyptus leaves, etc. Effective extraction of shikimic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Ginkgo biloba leaves were dried at 60°C, crushed and passed through a 60-mesh sieve, added water according to the material-to-water ratio of 1:20, leached in a water bath at 80°C for 3 hours, and after cooling to room temperature, centrifuged at 2000rpm to remove the residue. get the extract;

[0041] (2) Heat and concentrate the extract to 0.5 times the original volume, inoculate 1% rum yeast, and ferment for 6 days under anaerobic conditions at 30°C to obtain a fermentation broth;

[0042] (3) Add β-cyclodextrin solution with a mass concentration of 0.5% to the fermentation broth, and mix evenly to obtain the mixed solution 1, which is pasteurized for 50 minutes to obtain the mixed solution 2;

[0043] The volume ratio of the fermented broth and the β-cyclodextrin solution is 1:45;

[0044] (4) Add 1% activated carbon by volume to the mixture 2, decolorize at 70°C for 50 minutes, remove the activated carbon by filtration, and obtain a crude extract;

[0045] (5) C...

Embodiment 2

[0048] (1) Take liquidambar leaves and dry them at 60°C, crush them through an 80-mesh sieve, add water according to the material-to-water ratio of 1:30, extract in a water bath at 100°C for 4 hours, and centrifuge at 3000rpm to remove the residue after cooling to room temperature , to obtain the extract;

[0049] (2) Heat and concentrate the extract to 0.6 times the original volume, inoculate 3% rum yeast, and ferment for 8 days under anaerobic conditions at 35°C to obtain a fermentation broth;

[0050] (3) Add β-cyclodextrin solution with a mass concentration of 0.5% to the fermentation broth, mix evenly to obtain the mixed solution 1, and pasteurize it for 60 minutes to obtain the mixed solution 2;

[0051] The volume ratio of the fermented broth to the β-cyclodextrin solution is 1:55;

[0052] (4) Add 1.5% activated carbon by volume to the mixed liquid 2, decolorize at 80°C for 60 minutes, remove the activated carbon by filtration, and obtain a crude extract;

[0053] (5...

Embodiment 3

[0056] (1) Take lemon eucalyptus leaves and dry them at 60°C, crush them through a 70-mesh sieve, add water according to the material-to-water ratio of 1:25, extract in a water bath at 90°C for 4 hours, and centrifuge at 3000 rpm to remove the residue after cooling to room temperature , to obtain the extract;

[0057] (2) Heat and concentrate the extract to 0.6 times the original volume, inoculate 2% rum yeast, and ferment for 7 days under anaerobic conditions at 32°C to obtain a fermentation broth;

[0058] (3) Add β-cyclodextrin solution with a mass concentration of 0.5% to the fermentation broth, mix evenly to obtain the mixed solution 1, and pasteurize it for 60 minutes to obtain the mixed solution 2;

[0059] The volume ratio of the fermentation broth and the β-cyclodextrin solution is 1:50;

[0060] (4) Add 1.3% activated carbon by volume to the mixture 2, decolorize at 75°C for 60 minutes, remove the activated carbon by filtration, and obtain a crude extract;

[0061]...

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Abstract

The invention provides a shikimic acid extraction method. Shikimic acid is extracted from a natural substance such as star anise, cedrus, folium ginkgo, masson pine needles, folium liquidambaris and lemon-scented gum leaves. The shikimic acid is obtained by crushing raw materials, performing rough extraction with water, then inoculating with a sweet distiller yeast for fermentation, decomposing the raw materials into glucose by use of rhizopus and mucor, performing yeast fermentation by use of sugar to generate ethanol, adding a beta-cyclodextrin solution and the fermentation product ethanol for secondary extraction and performing decolorization, filtering and crystallization. The shikimic acid extraction process is simple and high in raw material utilization rate, and has good economic benefits.

Description

technical field [0001] The invention belongs to the technical field of extraction of natural active ingredients, in particular to a shikimic acid extraction method. Background technique [0002] Shikimic acid is also known as 3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid, molecular formula: C 7 h 10 o 5 , mainly exists in the dried fruit of Magnoliaceae star anise, and also exists in other higher plants and microorganisms, such as Bashan fir and Masson pine. Tamiflu, a substance derived from shikimic acid, has miraculous effects against influenza and has attracted widespread attention. Studies have found that in addition to this, shikimic acid has obvious antithrombotic effects, and also has convulsive effects on human nerves. [0003] Chinese invention patent CN200910039670.3, a method for extracting shikimic acid from star anise, first using supercritical CO 2 Fluid extraction of fennel oil in star anise; then soaking degreased star anise in water at room temperatu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C51/43C07C51/50C07C62/32
CPCC07C51/42C07C51/43C07C51/47C07C51/50C07C2601/16C07C62/32
Inventor 黄陈玉
Owner 南宁市黄陈生猪养殖场
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