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A kind of extract containing hollyn A and its extraction method

An extraction method, a technology of hollynin, which is applied in the production of steroids, organic chemistry, bulk chemicals, etc., can solve the problems of cumbersome separation and preparation of hollynin A, low purity, etc., and achieve the improvement of total triterpenoid components, extraction High yield and reduced separation difficulty

Active Publication Date: 2021-01-05
江苏海钥医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The first object of the present invention is to provide a kind of extraction method that contains the extract of hollyn A, described method can finish the extraction of hollyn A in a short period by resin-supercritical separation chromatography coupling technology, and purity can It reaches more than 70%, and at the same time, the content of impurities is reduced, which solves the problems of cumbersome separation and preparation process and low purity of hollyn A in the prior art

Method used

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  • A kind of extract containing hollyn A and its extraction method
  • A kind of extract containing hollyn A and its extraction method
  • A kind of extract containing hollyn A and its extraction method

Examples

Experimental program
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Embodiment 1

[0052] The extraction method described in the present embodiment comprises the following steps:

[0053] (1) Grind the leaves of Hainan holly through a 60-mesh sieve, add them to 55% ethanol aqueous solution, heat and reflux at 70-80°C according to the ratio of material to liquid at 1:6, repeat the extraction twice, each time for 2 hours, filter, and combine The filtrate was concentrated under reduced pressure until there was no smell of ethanol to obtain a concentrated solution.

[0054] (2) Add dilute hydrochloric acid to adjust the pH of the concentrated solution to 3, use HPD-100 macroporous adsorption resin as a filler, load the concentrated solution, and then use 20% ethanol aqueous solution and 80% ethanol aqueous solution for elution, each gradient elution 4 times the column volume, collect the eluate obtained by elution with 80% ethanol aqueous solution.

[0055] (3) Recover the eluate collected in step (2) to obtain a clear paste, add 1 times the amount of silica ge...

Embodiment 2

[0058] The present embodiment refers to the extraction method of Example 1, and the only difference is that the solvent used for extraction in step (1) is 60% aqueous ethanol.

Embodiment 3

[0060] The present embodiment refers to the extraction method of Example 1, the only difference being that the solvent used for extraction in step (1) is 50% aqueous ethanol.

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Abstract

The invention relates to the technical field of extraction of natural drugs and in particular relates to extract containing ilicin A and an extraction method thereof. The extraction method comprises the following steps: taking an ethanol solution as a solvent, and refluxing and extracting ilex hainanensis leaves; collecting an extracting solution and concentrating to obtain a concentrated solution; regulating the pH (Potential of Hydrogen) of the concentrated solution to be 2 to 3; taking macroporous adsorption resin as filler and carrying out elution on the concentrated solution by adopting the ethanol solution with the concentration of 10 to 30 percent and the ethanol solution with the concentration of 70 to 90 percent in sequence; recycling and concentrating an eluant obtained by eluting through the ethanol solution with the concentration of 70 to 90 percent to obtain clear extract; adding silica gel and uniformly blending; decompressing and drying to obtain blended sample powder; filling the blended sample powder into a silica gel column; carrying out supercritical elution; collecting an elution solution, crystallizing and drying to obtain the extract containing the ilicin A. The ilicin A is extracted, the purity can reach 70 percent or above and the content of total triterpenoids reaches 95 percent or above; the content of impurities is reduced and the problems in the prior art that a technology is complicated and the purity is low are solved.

Description

technical field [0001] The invention relates to the technical field of extraction of natural medicines, in particular to an extract containing hollylicin A and an extraction method thereof. Background technique [0002] Traditional Chinese medicine or natural medicine shows great potential in the prevention and treatment of malignant tumors with its unique curative effect. Hainan holly (Ilex hainanensis Merr.) is a Chinese herbal medicine with a long history of folk use in Guangxi Zhuang and Yao. It is mainly distributed in Guangdong, Guangxi, Hainan, Guizhou, In Yunnan and other places, it is recorded in "Guangxi Standards of Chinese Medicinal Materials", "Guangxi Zhuang Medicine Quality Standards", and the appendix of the National Pharmacopoeia. Folks usually drink it as tea. [0003] Hainan holly leaves mainly include flavonoids, caffeoylquinic acids, triterpene acids, triterpene saponins, volatile oils and organic acids. Among them, it has been found through research t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/008Y02P20/54
Inventor 王媛媛
Owner 江苏海钥医药科技有限公司