Reagent and electrochemical sensor

An electrochemical and sensor technology, applied in the field of reagents and electrochemical sensors, can solve the problems of low precision, inaccurate test results, interference of urine sugar test, etc., and achieve the effect of good accuracy

Pending Publication Date: 2018-12-25
上海荒岛科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, urine test paper is mostly used for clinical urine sugar detection. Urine test paper relies on the glucose in the urine to react with the enzyme on the test paper and change the color of the dye reagent. The change of color reflects the concentration of glucose, but its accuracy is low, and it is a semi-quantitative test.
In addition, there is a plan to test the concentration of urine sugar with electrochemical test strips, but the use of electrochemical test strips to test urine sugar is rare in the market. The main reason is that the substances in urine are complex and interfere seriously with urine sugar testing. , such as ascorbic acid, a large number of electrolyte ions and metal ions in urine with high urine specific gravity, will cause relatively large interference to the electrochemical enzyme sensor to test urine sugar, resulting in inaccurate test results

Method used

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  • Reagent and electrochemical sensor
  • Reagent and electrochemical sensor
  • Reagent and electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Such as figure 1 and figure 2 as shown, figure 1 and figure 2 It is an electrochemical sensor used in the embodiment of the present invention. In the figure, 1 is the substrate, 2 is the working electrode, 3 is the counter electrode, 4 is the trigger electrode, 5 is the insulating film, 6 is the reagent layer, and 7 is the hydrophilic film. , 8 is an adhesive layer (such as a double-sided adhesive layer), 9 is a sample guide groove, and 10 is an air outlet;

[0077] The surface of the base material is screen-printed with electrode shapes, which are L-shaped working electrode 2, L-shaped counter electrode 3 and T-shaped trigger electrode 4, and the trigger electrode 4 is arranged in the space formed by the working electrode 2 and the counter electrode 3;

[0078] The L end side surface of the working electrode 2 is provided with an insulating film 5, and the insulating film 5 has an opening, and the reagent layer 6 is disposed in the opening;

[0079] An adhesive l...

Embodiment 2

[0082] Detect urine urine specific gravity, adopt the sensor of embodiment 1, the reagent composition in the reagent layer in the sensor is as shown in table 1;

[0083] Table 1 The reagent data table of embodiment 2 reagent layer

[0084] substance

mass ratio

Potassium ferricyanide

6.45％

Trehalose

1.8％

Hydrophilic silica

0.9％

Tween-20

0.45％

Phosphate buffer solution

45.2％

water

45.2％

[0085] Take 10 urine samples, numbered 1-10 respectively, apply +0.4V voltage to the sensor with a measuring instrument, test the response current for 30s, and directly obtain the urine specific gravity from the measuring instrument end, based on the composition of the above reagents, the urine specific gravity and the electrode current The calculation formula is Y=0.00148*X+0.99922. image 3 Fitting a line plot for urine specific gravity versus electrochemical sensor test current. The formula is pre-set inside the ...

Embodiment 3

[0090] The process of testing the specific gravity of urine is the same as in Example 2, except that the reagent composition of the reagent layer is different, as shown in Table 3; the test results and comparison results are shown in Table 4; Figure 4 The urine specific gravity and the electrochemical sensor test current fitting line diagram provided for the present embodiment;

[0091] Table 3 The reagent data table of embodiment 3 reagent layer

[0092]

[0093]

[0094] Table 4 This embodiment obtains the urine specific gravity and the urine specific gravity error data table of the refractometer test

[0095]

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PUM

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Abstract

The invention provides a reagent and an electrochemical sensor, in particular, a reagent for detecting the specific gravity of urine. The reagent comprises: 2 to 10wt% of an electron mediator, 1 to 5wt% of a stabilizer, 0.5 to 2wt% of an anti-coagulant, and 0.1 to 0.5wt% of a surfactant; the balance is pH buffer and water, and the mass ratio of pH buffer to water is 1:1. The invention also provides a reagent for detecting urine glucose, which increases by 0.5 to 5wt% of a glucose oxidase or a glucose dehydrogenase on the basis of the reagent for detecting the urine specific gravity. The reagent has a high accuracy in detecting the urine specific gravity or the urine glucose by selecting a specific proportion of components and controlling the content of each component.

Description

technical field [0001] The invention relates to the technical field of urine detection, in particular to a reagent and an electrochemical sensor. Background technique [0002] Urine routine is a basic routine item for disease detection. Tests such as urine specific gravity and urine sugar in urine routine are routine inspection items. The urine sugar detection is taken as an example for description below. [0003] Urine sugar (urine glucose) examination is the simplest method for early diagnosis of diabetes. Urine sugar will appear after the blood sugar concentration exceeds the renal sugar threshold. The higher the blood sugar concentration, the higher the urine sugar concentration. As a simple and non-invasive detection method, urine glucose detection is a very beneficial method for diabetic patients. [0004] At present, urine test paper is mostly used for clinical urine glucose detection. Urine test paper relies on the glucose in the urine to react with the enzyme on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N9/00G01N9/36
CPCG01N9/00G01N9/36G01N27/3272
Inventor 王文冲徐晨龙
Owner 上海荒岛科技有限公司
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