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Method for lignocellulose one-pot bioconversion

A technology of lignocellulose and biotransformation, applied in the fields of biotransformation of lignocellulose and whole-bacteria saccharification of lignocellulose, can solve the problems of poor technical matching, high cost, serious pollution, etc., to reduce the cost of using enzymes, reduce Cost of carbon source, effect of simple process

Active Publication Date: 2018-12-28
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the existing problems in lignocellulose utilization such as cumbersome steps, poor technology matching, high cost and serious pollution, the present invention provides a one-pot biotransformation method of lignocellulose, which has the advantages of low cost and simple process, etc. Advantages, and reduce waste water discharge, good prospects for industrial application

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Examples

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Embodiment 1

[0029] Embodiment 1: Lignocellulose one-pot bioconversion method

[0030] A method for one-pot biotransformation of lignocellulose, comprising the following steps:

[0031] (1) Mechanical treatment: mechanically pulverize the corn stalks until the particle size of the fragments does not exceed 2cm; wash with water, then put it into the closed reactor from the feeding port above the closed reactor, and add it according to the solid-liquid weight volume ratio of 1:2 water, mix evenly, and soak for 4 hours.

[0032] (2) Chemical treatment: Ammonium sulfite was added into the closed reactor until its final concentration was 11% (w / v), and sulfonation treatment was carried out at 175° C. for 3.5 hours. After the reaction is completed, the discharge port at the bottom of the closed reactor is opened to allow the black liquor to flow out into the storage tank through the filter device equipped at the discharge port; the storage tank is connected to the closed reactor through a pipel...

Embodiment 2

[0039] Embodiment 2: Lignocellulose one-pot bioconversion method

[0040] Different from Example 1, the method for one-pot bioconversion of lignocellulose comprises the following steps:

[0041] (1) Mechanical treatment: Mechanically pulverize the wheat straw until the particle size of the fragments does not exceed 2cm; wash with water, then put it into the closed reactor from the feeding port above the closed reactor, and add water according to the solid-liquid weight volume ratio of 1:3 , mix evenly, soak for 2-4h.

[0042] (2) Chemical treatment: Ammonium sulfite was added into the closed reactor until its final concentration was 12% (w / v), and sulfonation treatment was carried out at 180° C. for 4 hours. After the reaction is completed, the black liquor is flowed into the storage tank until the water content of the material in the closed reactor reaches 30%-50%, and the discharge port is closed.

[0043] (3) Material cleaning: add water of 1 times the material volume in ...

Embodiment 3

[0049] Embodiment 3: Lignocellulose one-pot bioconversion method

[0050] Different from Example 1, the method for one-pot bioconversion of lignocellulose comprises the following steps:

[0051] (1) Mechanical treatment: Mechanically crush the shrub branches until the particle size of the fragments does not exceed 2cm; wash with water, and then put it into the closed reactor from the feeding port above the closed reactor, according to the solid-liquid weight volume ratio of 1:4 Add water, mix well, soak for 2-4h.

[0052] (2) Chemical treatment: Ammonium sulfite was added into the closed reactor until its final concentration was 13% (w / v), and sulfonation treatment was carried out at a temperature of 185° C. for 1 hour. After the reaction is completed, the black liquor is flowed into the storage tank until the water content of the material in the closed reactor reaches 30%-50%, and the discharge port is closed.

[0053] (3) Material cleaning: add water of 1.5 times the mater...

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Abstract

The invention provides a method for lignocellulose one-pot bioconversion. The method comprises the following steps: (1) mechanical treatment: wood lignocellulose raw materials are crushed, cleaned andput into a closed reactor, and water is added for soaking; (2) chemical treatment: ammonium sulfite is added into the reactor, and black liquor is allowed to drain out after sulfonation treatment iscompleted; (3) material cleaning: water is added into the reactor and mixed evenly, and high pressure nitrogen is fed for draining out the black liquor; (4) saccharification: a saccharification mediumis added into the reactor, and sterilization is performed under high temperature and high pressure, cellulosome-producing strain seed liquid is inoculated into the reactor, and liquid glucose containing glucose is obtained after saccharification; and (5) fermentation: activated aerobic heterotrophic microbial seed liquid is inoculated into an obtained fermentation medium for fermentation. By adopting the method provided by the invention, the process steps and the requirements for equipment are simplified, the costs in carbon sources and enzymes consumption are reduced, at the same time, the problem of comprehensive utilization of agricultural and forestry waste is solved, and the method has great environmental benefits and social benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a biotransformation method of lignocellulose, in particular to a whole-bacteria saccharification method for lignocellulose. Background technique [0002] In recent years, obtaining renewable energy, materials or chemicals from biomass has attracted widespread attention from all over the world. The use of lignocellulosic biomass as raw materials to produce second-generation bio-based fuels or chemicals has become the key to large-scale replacement of petroleum-based products. Lignocellulosic biomass is the most abundant renewable biomass resource on earth, and its conversion into energy, chemicals or materials has great application and development prospects. In particular, my country is a big agricultural country, with an annual output of more than 900 million tons of crop straw. On the other hand, problems such as environmental pollution and traffic safety accidents caused by straw b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/14
CPCC12P19/02C12P19/14
Inventor 崔球刘亚君李滨冯银刚
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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