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Extraction process for tea polysaccharide of moringa oleifera

An extraction process, the technology of Moringa oleifera tea, is applied in the field of extraction process of polysaccharides of Moringa oleifera tea, which can solve the problems of easy destruction of polysaccharide structure, long extraction time of polysaccharides, and reduced bioavailability, so as to achieve short extraction time, low cost, The effect of accelerating dissolution

Pending Publication Date: 2019-01-04
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid and alkali methods are easy to destroy the structure of polysaccharides and reduce their bioavailability; the cost of enzyme extraction is high; simple water extraction method is difficult to diffuse polysaccharides from the cells and the extraction time is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of extraction technology of tea polysaccharide of Moringa oleifera, comprises the following steps:

[0041] (1) Pretreatment of raw materials: first pulverize the Moringa oleifera tea to obtain tea powder, then put the tea powder into an extruder and add 7% water by weight to squeeze to obtain tea dregs and tea liquid for subsequent use;

[0042] (2) Primary leaching: put the tea dregs into water at 80°C with a liquid-to-solid ratio of 60mL / g, extract with ultrasonic waves with a frequency of 45kHz for 105min, filter to obtain a primary filter residue and a primary filtrate, and set aside;

[0043] (3) Secondary leaching: put the primary filter residue into water at 25°C, the liquid-to-material ratio is 60mL / g, and add 0.08% of the filter residue weight to be composed of cellulase and pectinase with a mass ratio of 3:1 Mixing enzymes, extracting for 60 minutes with ultrasonic waves with a frequency of 45kHz, filtering to obtain secondary filter residue and second...

Embodiment 2

[0051] A kind of extraction technology of tea polysaccharide of Moringa oleifera, comprises the following steps:

[0052] (1) Pretreatment of raw materials: first pulverize the Moringa oleifera tea to obtain tea powder, then put the tea powder into an extruder and add 6% of its weight of water to squeeze to obtain tea dregs and tea liquid for subsequent use;

[0053] (2) Primary leaching: put the tea dregs into water at 60°C, with a liquid-to-solid ratio of 50mL / g, extract with an ultrasonic wave at a frequency of 48kHz for 80min, filter to obtain a primary filter residue and a primary filtrate, and set aside;

[0054] (3) Secondary leaching: put the primary filter residue into water at 28°C, the liquid-to-solid ratio is 70mL / g, and add 0.07% of the filter residue weight to be composed of cellulase and pectinase with a mass ratio of 3:1 Mixing enzymes, extracting with ultrasonic frequency of 48kHz for 45min, filtering to obtain secondary filter residue and secondary filtrate, ...

Embodiment 3

[0062] A kind of extraction technology of tea polysaccharide of Moringa oleifera, comprises the following steps:

[0063] (1) Pretreatment of raw materials: first pulverize the Moringa oleifera tea to obtain tea powder, then put the tea powder into an extruder and add 8% water by weight to squeeze to obtain tea dregs and tea liquid for subsequent use;

[0064] (2) Primary leaching: put the tea dregs into water at 70°C, with a liquid-to-solid ratio of 40mL / g, extract with an ultrasonic wave at a frequency of 42kHz for 135min, filter to obtain a primary filter residue and a primary filtrate, and set aside;

[0065] (3) Secondary leaching: put the primary filter residue into water at 22°C, the liquid-to-solid ratio is 30mL / g, and add 0.09% of the filter residue weight to be composed of cellulase and pectinase with a mass ratio of 3:1 Mix the enzymes, use ultrasonic frequency of 43kHz to extract 75min, filter to obtain the secondary filter residue and secondary filtrate, and the f...

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Abstract

The invention discloses an extraction process for tea polysaccharide of moringa oleifera and belongs to the biotechnical field. The extraction process comprises the following steps: first, carrying out pre-treatment of crushing and juicing a fresh moringa oleifera tea raw material; then carrying out secondary digestion; then carrying out impurity removal and ultrafiltration treatment on the obtained mixed solution; and carrying out concentration, alcohol precipitation and drying to obtain the tea polysaccharide of moringa oleifera. The extraction process for tea polysaccharide of moringa oleifera disclosed by the invention is simple, short in extraction time, low in cost and free of toxic and organic solvents, the structure of tea polysaccharide is not damaged, a relatively high tea polysaccharide extraction rate is acquired, and the extraction process is suitable for industrial production and application of tea polysaccharide of moringa oleifera.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an extraction process of tea polysaccharides from Moringa oleifera. Background technique [0002] my country is a big country in tea production, but the total output value of tea production is not high, because most of the tea has not been further processed. The only way to solve this problem is to make comprehensive use of it and extract its active ingredients. Tea polysaccharide is another important physiologically active substance discovered after tea polyphenols. It is an acidic glycoprotein combined with a large amount of mineral elements. Its composition is related to the type of tea and the extraction method. Tea polysaccharide has many biological activities such as lowering blood pressure, lowering blood sugar, lowering blood fat, enhancing immunity, etc. It is a promising natural medicine. [0003] Moringa, a plant of the genus Moringa in the Moringaceae family...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张秀芬莫周美刘连军李恒锐谢君锋马仙花韦雪英黎萍杨海霞梁振华何文林嘉雯黄珍玲郭素云符策
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI