Method for producing single cell protein through double-bacteria step fermentation taking ethyl alcohol as transition carbon source

A single-cell protein, step-by-step fermentation technology, applied in the field of double-bacteria step-by-step fermentation to produce single-cell protein, can solve the problems of long fermentation cycle and low carbon source utilization efficiency, and achieves improved utilization efficiency, slow down catabolism inhibition, production The effect of speed increase

Inactive Publication Date: 2019-01-04
NANJING FORESTRY UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Purpose of the invention: When Candida utilis uses mixed sugar (glucose and xylose) as carbon source to ferment single-cell protein, due to the influence of carbon source catabolism and Crabtree effect, Candida utilis preferentially uses glucose to generate ethanol, and secondly Using the ethanol produced by itself, and finally reusing xylose, leads to the problems of low carbon source utilization efficiency and too long fermentation period. The method of cell protein, in order to improve the utilization efficiency of carbon source in the case of multiple carbon sources, and improve the yield of single cell protein

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  • Method for producing single cell protein through double-bacteria step fermentation taking ethyl alcohol as transition carbon source
  • Method for producing single cell protein through double-bacteria step fermentation taking ethyl alcohol as transition carbon source
  • Method for producing single cell protein through double-bacteria step fermentation taking ethyl alcohol as transition carbon source

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Embodiment 1

[0034] Double-bacteria step-by-step fermentation to produce single-cell protein, the steps are as follows:

[0035] (1) Using mixed sugar as a carbon source, add nitrogen source and inorganic salts in Pichia stipitis fermentation medium, after sterilization, insert Pichia stipitis CBS 5776 into a constant temperature shaker culture, 30°C, 150r / min, ferment for 24-36 hours; the ventilation method is in the form of non-baffled Erlenmeyer flask and cotton plug. The fermentation broth was centrifuged to obtain the bacteria and ethanol-containing supernatant. The precipitate (thalline) obtained by centrifugation is washed and dried as single-cell protein; the resulting ethanol-containing supernatant is used for the production of single-cell protein by Candida utilis. Among them, Pichia stipitis needs to be activated for 36 hours and after a round of proliferation (24 hours), it is inserted into the fermentation, and the initial OD of the fermentation broth inserted into Pichia st...

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Abstract

The invention discloses a method for producing a single cell protein through double-bacteria step fermentation taking ethyl alcohol as a transition carbon source. The method comprises the following steps that mixed saccharides are used as a carbon source, pichia stipitis is taken as a fermentation strain, a pichia stipites single cell protein and a fermentation liquid containing the ethyl alcoholare prepared through fermentation, then candida utilis is added into the fermentation liquid, and a candida utilis single cell protein is prepared through fermentation. The ethyl alcohol is taken as the middle transition carbon source, the single cell protein is produced through the double-bacteria step fermentation, the result finds that the double-bacteria step fermentation can better solve theproblem of mutual inhibition of two thalli under the co-fermentation condition of the mixed bacteria, and compared with the single-bacterium fermentation of the candida utilis and the pichia stipites,the production rates of a crude protein in the double-bacteria step fermentation are relatively obviously increased by 102.72% and 38.41% respectively.

Description

technical field [0001] The invention relates to the technical field of producing single-cell protein (feed protein) and other biological products through biotransformation of agricultural waste, in particular to a method for producing single-cell protein through double-bacteria step-by-step fermentation using ethanol as a transitional carbon source. technical background [0002] Since the 20th century, with the continuous development of science and technology, human beings are also facing various challenges while enjoying the convenience brought by science and technology. The three major problems of energy shortage, climate change and environmental pollution are becoming more and more serious. Among them, the energy problem is particularly urgent. How to develop and utilize renewable new energy has become one of the focuses of research in various countries. At the same time, in the traditional feed protein industry, the original feed production can no longer meet the needs o...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/84C12R1/72
CPCC12N1/16
Inventor 朱均均伍陆李欢徐勇李鑫余世袁
Owner NANJING FORESTRY UNIV
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