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Application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation

A Bacillus subtilis, cellulose degradation technology, applied in the application, soil conditioning materials, chemical instruments and methods, etc., can solve problems such as direct burning or discarding, waste of resources, environment, pollution, etc.

Active Publication Date: 2019-01-04
云南星耀生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complex structure of hemicellulose and lignin in plant cell walls and their encapsulation of cellulose limit the large-scale application of straw resources in animal husbandry production
At present, the actual use of straw in industry and feed production accounts for about 30% of the total, and most of the rest are directly burned or discarded, resulting in a great waste of resources and environmental pollution

Method used

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  • Application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation
  • Application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation
  • Application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation of embodiment 1 bacillus subtilis (Bacillus subtilis) XF-1 bacterial agent

[0015] This example introduces the preparation of Bacillus subtilis (Bacillus subtilis) XF-1 bacterial agent.

[0016] 1. Preparation of Bacillus subtilis XF-1 liquid preparation

[0017] Activation of Bacillus subtilis (Bacillus subtilis) XF-1: From the slant of Bacillus subtilis (Bacillus subtilis) XF-1, use a sterile inoculation needle to pick part of the bacterial lawn into LB shake flasks (LB liquid culture in 250mL shake flasks 50ml of base solution), after culturing at 30°C and 150r / min for 24h, the LB medium became turbid. Take the bacterial liquid and streak it into the LB solid medium for 24h-36h. Bacillus subtilis XF-1 grows well on the LB solid medium. The colonies are white or off-white, with smooth edges, irregular surface, and opaque.

[0018] Preparation of seed solution: the liquid volume of LB liquid medium in the 500mL seed shake flask is 150ml. Pick a sing...

Embodiment 2

[0028] The salt tolerance of embodiment 2 bacillus subtilis (Bacillus subtilis) XF-1

[0029] Basal medium: glucose 20g / L, yeast extract 5g / L, pH 7-7.5.

[0030] By adding different amounts of sodium chloride in the basal medium, the final concentration of sodium chloride was 0g / L, 5g / L, 10g / L, 15g / L, 20g / L, 25g / L and 30g / L respectively. liquid medium.

[0031] Shake flask culture conditions: the filling volume of the liquid culture medium in the 250mL shake flask is 50ml. Inoculum volume: 1mL seed solution / shake flask. Cultivate on a shaker at 30°C and 150r / min for 72 hours, and take samples every 12 hours to determine the content of viable bacteria.

[0032] Table 1 Bacillus subtilis XF-1 cultivated live bacteria content (10 8 cfu / mL)

[0033]

[0034] It can be seen from Table 1 that Bacillus subtilis (Bacillus subtilis) XF-1 can grow normally under the salt concentration of 25g / L, and has high salt tolerance.

[0035]In the process of utilization, saline-alkali la...

Embodiment 3

[0037] The phosphorus solubilizing effect of embodiment 3 bacillus subtilis (Bacillus subtilis) XF-1

[0038] 1. Determination of the phosphorus-solubilizing effect of Bacillus subtilis by plate lysing zone method

[0039] Phosphorus solubilizing ability is an important index to characterize the phosphorus solubilizing effect of microorganisms. Generally, both qualitative and quantitative methods can be used.

[0040] way.

[0041] The qualitative method generally refers to the plate lysate zone method. Plate lysis zone method: culture phosphate-dissolving strains on a solid plate medium containing insoluble phosphate, and measure the size of the transparent zone produced by the colony. The ratio (D / d) of the diameter of the phosphorus-dissolving circle (D) to the diameter of the colony growth (d) was used to characterize the relative phosphorus-solubilizing ability of the phosphorus-solubilizing bacteria.

[0042] The medium used in the plate lysing zone method is Montkina...

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Abstract

The invention provides application of bacillus subtilis in soil phosphorus decomposition and cellulose degradation, relates to the technical field of microbial pesticides, and particularly provides application of bacillus subtilis XF-1 in the soil phosphorus decomposition and the cellulose degradation. The bacillus subtilis XF-1 has remarkable phosphorus decomposing and cellulose degrading functions, can tolerate a certain degree of salinity and can survive in a 2.5% of sodium chloride solution, and accordingly a bacillus subtilis XF-1 fungicide can be used in salinity soil.

Description

technical field [0001] The invention relates to the field of microbial pesticides, and more specifically relates to the application of bacillus subtilis in soil phosphorus solubilization and cellulose degradation. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth. It is easily fixed in the soil and its effective utilization rate is reduced. Using phosphorus-dissolving microorganisms to convert phosphorus that is difficult for plants to absorb into a form that is easily absorbed and utilized in the soil is an important way to improve the utilization rate of phosphorus in soil. In addition, the development and utilization of salinized soil such as coastal tidal flats and the solution of soil compaction caused by continuous cropping are very urgent and important tasks in my country's agricultural production. The use of microorganisms capable of dissolving phosphorus can not only effectively improve the utilization of phosphorus in the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C09K17/14C09K101/00C09K109/00C12R1/125A62D101/28A62D101/45
CPCA62D3/02A62D2101/28A62D2101/45C09K17/14C09K2101/00C09K2109/00C12N1/20
Inventor 仇俊涛何月秋罗树荣
Owner 云南星耀生物制品有限公司
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