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Fluorescent sensor based on carbon nitride desorption and preparing method and application thereof

A fluorescent sensor, carbon nitride technology, applied in the field of fluorescent sensors, can solve the problems of cumbersome and expensive operations, and achieve high sensitivity

Inactive Publication Date: 2019-01-04
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

HIV nucleic acid quantitative detection and quantitative methods generally include two methods: RT-PCR and signal amplification amplification, which usually require the use of expensive instruments and are more cumbersome to operate

Method used

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  • Fluorescent sensor based on carbon nitride desorption and preparing method and application thereof
  • Fluorescent sensor based on carbon nitride desorption and preparing method and application thereof
  • Fluorescent sensor based on carbon nitride desorption and preparing method and application thereof

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preparation example Construction

[0042] A fluorescent sensor based on the desorption of carbon nitride prepared by the above-mentioned preparation method.

[0043] An application of the above-mentioned fluorescent sensor based on carbon nitride desorption for the detection of HIV nucleic acid small molecules.

[0044] Furthermore, when used for the detection of HIV nucleic acid small molecules, the fluorescent sensor based on carbon nitride desorption is used as a substrate, and the system to be measured is added to the photoelectrochemical sensor based on carbon nitride signal amplification. In the sensor, ultraviolet lamps are combined for illumination and chemiluminescence fluorescence imaging system imaging. In a system where HIV exists, the fluorescence sensor based on carbon nitride desorption will produce enhanced luminous intensity. By drawing known HIV nucleic acid small The standard curve of molecular concentration and luminous intensity, the luminous intensity detected by the fluorescent sensor bas...

Embodiment 1

[0061] A method for preparing a fluorescent sensor based on carbon nitride desorption to measure HIV nucleic acid small molecules, comprising the following steps:

[0062] (1) Establishment of standard curve:

[0063] ①The chemiluminescent reagents MCLA, g-C 3 N 4 1. The complementary strand of HIV-DNA is mixed with equal volumes of HIV-DNA standard solutions of different concentrations, and Tris-HCl buffer is added to prepare a series of reagents to be tested;

[0064] ②Add the reagent to be tested into the microtiter plate, and after irradiating for 60s under a 365 nm ultraviolet lamp, place it in the darkroom of the chemiluminescence fluorescence imaging system, and the exposure time for each time is 90s;

[0065] ③ Through software reading and conversion, obtain the luminous intensity corresponding to different concentrations of HIV-DNA standard solutions;

[0066] ④ Draw the value obtained in step ③ into a standard curve, and the linear regression equation is: y=1.622 ...

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Abstract

The invention discloses a fluorescent sensor based on carbon nitride desorption and a preparing method and application thereof. The preparing method for the sensor includes the steps that a g-C3N4 nanosheet serves as a reinforcing agent of a chemiluminesence reagent, and a complementary chain of to-be-detected biomolecule DNA serves as a biological recognition element; the g-C3N4 nanosheet, the chemiluminesence reagent, the complementary chain of the to-be-detected biomolecule DNA and a Tris-HCI buffer solution are mixed, and the fluorescent sensor based on carbon nitride desorption is prepared. The sensor has the advantages of being simple, convenient to use, accurate and the like; when the fluorescent sensor is used for detecting HIV nucleic-acid small molecules, the linearity range is 50 nM to 2,000 nM, and the detection limit (3 S / N) is 20 nmol / L.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection, and in particular relates to a fluorescence sensor based on carbon nitride desorption and its preparation and application. Background technique [0002] AIDS is a very harmful infectious disease caused by HIV infection. After HIV invades the human body, some people will show no obvious symptoms for a period of time, that is, the incubation period is different for each person. Therefore, HIV infection detection is very important for the prevention and treatment of HIV. Under normal circumstances, after the virus infects the human body, it can be detected through a series of tests, and the first thing that can be detected is the viral nucleic acid. Nucleic acid detection can directly check HIV nucleic acid, detect HIV infection before serological changes are found, and is more sensitive than P24 antigen detection method, and can detect viral infection earlier. Quantitative detecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486
Inventor 王建江舟张悦
Owner FUZHOU UNIV
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