Method for obtaining selfing progeny of stevia rebaudianum through embryo rescue

An embryo rescue and stevia technology, applied in the field of biotechnology breeding, can solve the problems of low self-seeding rate of stevia

Active Publication Date: 2019-01-08
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a method for obtaining stevia self-fertilization progeny through embryo rescue, which effectively overcomes the incompatibility obstacle of stevia self-fertilization embryo abortion and solves the problem of low self-fertilization rate of stevia

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  • Method for obtaining selfing progeny of stevia rebaudianum through embryo rescue

Examples

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Embodiment 1

[0027] A method for obtaining selfed offspring of Stevia through embryo rescue, comprising the following steps: (1) preparation of parent material: the parent selects a cultivar 'Zhongshan No. 8' with high content of total steviol glycosides and good comprehensive traits. The embryo rate is as high as 16%, but the natural self-fertilization rate is 0 due to the abortion of immature embryos. In May 2017, 'Zhongshan No. 8' was propagated by cuttings, rooted and transplanted in flowerpots, and managed with conventional water and fertilizer;

[0028] (2) Selfing: After removing the open flowers when Stevia 'Zhongshan No. 8' blooms, use sulfuric acid paper bagging to isolate, and then carry out artificial assisted pollination every other day; the artificial assisted pollination is to uncover the same species The inflorescences of the sulfuric acid paper bag approached and rubbed against each other, spreading pollen to the stigma;

[0029] (3) Stripping of ovules: select flower head...

Embodiment 2

[0034] The method of obtaining the selfed offspring of Stevia through embryo rescue described in this example is generally the same as that of Example 1, the difference is that: (1) the parent material is a Stevia variety 'Zhongshan No. 5' with a higher stevioside content; (2) the The self-crossing was carried out under geographical isolation conditions. 30 plants of 'Zhongshan No. 5' were selected and propagated by cuttings, and planted in the field, 5 plants x 6 rows, with a plant spacing of about 30cm. There is no stevia planting within 20km nearby, and topping after transplanting promotes Branches; (3) the artificial assisted pollination is in the flowering period of 'Zhongshan No. 5' from late August to early September, at 10-11 am every day, stick the upper end of the plant with a stick and gently rub it horizontally, vibrate the inflorescence, and make the pollen scattered (4) The stripping of the ovule is to collect the capitulum about 10 days after flowering and bring ...

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Abstract

The invention belongs to the field of biotechnology breeding and in particular relates to a method for obtaining selfing progeny of stevia rebaudianum through embryo rescue. The method specially comprises the following steps: selecting a stevia rebaudianum variety that selfing seeds cannot be obtained because of young embryo abortion, as a parent; carrying out artificial supplementary pollinationand selfing; peeling off young embryos 5-7 days after pollination; and carrying out in-vitro culture on the young embryos so as to obtain seedlings S1; transplanting the seedlings S1, thereby obtaining complete plants. By adopting an embryo rescue technique, the self-incompatibility obstacle of stevia rebaudianum is overcome, selfing progeny of the stevia rebaudianum is obtained, a part of properties are homozygoted, homozygote and a selfing line can be obtained through multiple generations of selfing, the breeding efficiency of the stevia rebaudianum can be effectively improved, and the stevia rebaudianum variety breeding progress can be accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for obtaining selfed offspring of stevia through embryo rescue. Background technique [0002] Stevia (Stevia rebaudiana Bertoni) belongs to the Asteraceae Stevia genus and is an emerging sugar crop. The steviol glycosides contained in its leaves have high sweetness and low calories (the sweetness is more than 300 times that of sucrose, and the calories are only 1 / 300 of sucrose) It is a natural low-calorie sweetener, widely used in food, beverage, medicine, chemical industry and other fields. The research on stevia germplasm resources and the cultivation of new fine varieties are very important to the development of stevia industry. However, due to the self-incompatibility of stevia, its natural self-seed setting rate is only 0.5%. Long-term hybridization leads to complex genetic background and severe separation of sexual reproduction offspring, which b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 杨永恒徐晓洋张婷孙玉明侯孟兰张永侠王银杰刘清泉原海燕
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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