Application of alternaria tenuissima in control of invasive alien weeds xanthium italicum

A technology of cocklebur and alternaria, which is applied in the fields of application, chemicals for biological control, herbicides and algicides, and can solve the problems of single targeting, time-consuming and labor-intensive, environmental pollution, etc.

Inactive Publication Date: 2019-01-08
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial control can only work in a short period of time and on a small scale, and it is time-consuming and laborious; chemical herbicides are prone to produce antibodies because of their single target, and because of the lack of microorganisms

Method used

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  • Application of alternaria tenuissima in control of invasive alien weeds xanthium italicum
  • Application of alternaria tenuissima in control of invasive alien weeds xanthium italicum
  • Application of alternaria tenuissima in control of invasive alien weeds xanthium italicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation, purification and screening of pathogenic bacteria of Xanthium fruticosa:

[0037] Isolation and purification of the pathogenic bacteria of Xanthium fruticosa:

[0038] Isolation of strains: Select the leaves or stems of Xanthia coxanthiae with typical lesions, rinse them with clean water, cut out 2mm×2mm tissue pieces at the junction of disease and health, and place them in 70% of the ultra-clean workbench. Alcohol, sterilized in 0.1% mercuric chloride, and then rinsed continuously in sterilized water for 3 times, after taking it out, absorb the excess water with sterilized absorbent paper and put it on a potato sucrose medium (PDA) plate, at a temperature of 25 ℃ Cultivated in a constant temperature incubator;

[0039] When the colony on the PDA plate medium grows to a certain size, it is separated and purified, and after pathogenicity determination, it is transferred to the slant of a potato sucrose medium (PDA) test tube and stored in a refrigerator at 4°...

Embodiment 2

[0056] The isolation, purification and screening of Xanthium cockleburi pathogenic bacteria are based on Example 1;

[0057] Extraction of crude toxin of Alternaria tennatus (XIF10):

[0058] Toxin-producing culture of XIF10 XIF10:

[0059] On the ultra-clean workbench, take out the well-cultured Alternaria Xanthii strain plate, pick a small amount of mycelium, inoculate it in potato dextrose (PDB) medium, put the inoculated potato dextrose (PDB) into the shaker Bed shaking culture, set the temperature at 25°C, rotate at 110r / min, alternate with darkness for 12 hours, and culture continuously for 7 days to obtain the fermentation broth;

[0060] Crude toxin extraction

[0061] The fermented liquid obtained was filtered once with four layers of gauze, the obtained filtrate was filtered with double-layer chromatography filter paper, and then the filtrate was centrifuged for 20 min at a speed of 10000 r / min, and the obtained supernatant was the toxin crude extract;

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Abstract

The invention relates to application of alternaria tenuissima in control of invasive alien weeds xanthium italicum. The pathogenic strains are classified and named as alternaria tenuissima, and the complete name is alternaria tenuissima. The pathogenic bacteria are separated and purified from leaves of natural infection plants of xanthium italicum from suburban wild population in Urumqi, Xinjiang.Pathogenic hyphae suspension and crude toxins are respectively prepared from the separated and purified fungi alternaria tenuissima by adopting the conventional method, and the prepared pathogenic hyphae suspension and crude toxins are respectively uniformly sprayed on xanthium italicum plants and achieve excellent pathogenic effects and control effects on worst weeds xanthium italicum. The culture operation is simple, the culture medium material is readily available, the prepared hyphae suspension and toxins have high stability, the preparation process is simple, and volume production can berealized; the alternaria tenuissima is sprayed onto stems and leaves, is convenient to use, is capable of effectively killing the target weeds xanthium italicum, and has high environmental safety.

Description

technical field [0001] The invention belongs to the field of plant protection, and specifically relates to the application of Alternaria tennatus in the prevention and control of alien invasive weeds Xanthium italiana. Background technique [0002] Invasion of alien organisms is a worldwide problem. Invasive organisms reduce biodiversity by replacing the original species or inhibiting the survival of new species in the invaded area, thereby destroying the stability of the ecosystem and affecting the local economic development. my country is one of the countries most seriously harmed by biological invasion. Especially in recent years, with the rapid development of economy and the increasing prosperity of international trade, some alien species have quietly entered our country through commerce, tourism and other channels. According to statistics, there are currently more than 600 species of alien invasive plants in my country, more than 50 of which are listed among the 100 mo...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P13/00C12R1/645
CPCA01N63/30C12N1/14
Inventor 邵华魏彩霞王威张弛韩彩霞
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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