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Polypeptide with function of repairing oxidative damage, preparation method and application thereof

A technology of oxidative damage and function, applied in the preparation method of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., to inhibit the production of angiotensin-converting enzymes, broad market prospects, and inhibit the rise of blood sugar Effect

Active Publication Date: 2019-01-11
山东科桥生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports related to the active ingredients of snails. Only early studies have found that snail saliva contains tetramine, and histamine and choline derivatives have neuroactivity.

Method used

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  • Polypeptide with function of repairing oxidative damage, preparation method and application thereof
  • Polypeptide with function of repairing oxidative damage, preparation method and application thereof
  • Polypeptide with function of repairing oxidative damage, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A method for extracting a polypeptide having the function of repairing oxidative damage, the steps are as follows:

[0062] (1) After the fragrant snails are shelled, take the meat part, grind it, add 8 times the weight of acidic ethanol, the acidic ethanol is an ethanol solution with a volume concentration of 55%, and the acidic ethanol is adjusted to pH 4.8 with acetic acid, and stirred at 32°C Extract under the conditions for 2.5h, filter, take the filtrate, let it stand in an ice bath for 30min, take the supernatant, and remove the ethanol in the solution by rotary evaporation at 40°C, and then add diatoms in a proportion of 0.5% by mass Soil-carboxymethyl starch sodium complex (mass ratio 3:2), let stand at room temperature for 3 hours, centrifuge, take the supernatant, add 0.8 times the volume of hexane for extraction, retain the aqueous phase, and extract continuously for 4 times to obtain the polypeptide mixed stock solution;

[0063] (2) Separate the polypepti...

Embodiment 2

[0067] Using the Fmoc solid-phase synthesis method described by Liu Zhennan et al. (Liu Zhennan, Huang Qiang. Fmoc solid-phase synthesis method. Journal of Guangxi University for Nationalities, 1999, 5(2): 110-112), the artificially synthesized amino acid sequence is as shown in SEQ ID NO.1 indicated polypeptide.

Embodiment 3

[0069] A method for extracting a polypeptide having the function of repairing oxidative damage, the steps are as follows:

[0070] (1) After shelling the snails, take the meat part, grind it, add 12 times the weight of acidic ethanol, the acidic ethanol is an ethanol solution with a volume concentration of 45%, the acidic ethanol is adjusted to pH 5.2 with acetic acid, and stirred at 28°C Extract under conditions for 7 hours, filter, take the filtrate, let it stand in an ice bath for 30 minutes, take the supernatant, and remove the ethanol in the solution by rotary evaporation at 38°C, and then add diatomaceous earth in a proportion of 1% by mass -Sodium carboxymethyl starch complex (mass ratio 3:2), let stand at room temperature for 1.5h, centrifuge, take the supernatant, add 1.2 times the volume of hexane for extraction, retain the aqueous phase, and extract continuously twice to obtain the polypeptide mixed stock solution;

[0071] (2) Separate the polypeptide mixed stock ...

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Abstract

The invention relates to a polypeptide with a function of repairing oxidative damage, a preparation method and an application thereof. The polypeptide with the function of repairing oxidative damage has an amino acid sequence shown as SEQ ID NO.1. The invention also discloses the application of the polypeptide as a medicinal composition in preparing drugs for treating diseases caused by oxidativedamage or as a healthcare component in preparing antioxidant healthcare products. The invention first discloses a polypeptide compound containing 10 amino acid residues extracted from neptune shell. Through detection, the polypeptide compound is capable of restraining the generation of angiotensin converting enzyme in blood vessels, restraining blood glucose increase and repairing oxidative stressinjury caused by peroxide by eliminating the generation of in vivo ROS, can be used for developing drugs for subsequently treating diseases caused by oxidative damage and antioxidant healthcare products, and has a broad market prospect.

Description

technical field [0001] The invention relates to a polypeptide with the function of repairing oxidative damage and its preparation method and application, belonging to the technical field of functional polypeptides. Background technique [0002] At present, the top three chronic non-communicable diseases (NCDs) in terms of mortality and morbidity in developed and developing countries are diabetes, cancer, cardiovascular and cerebrovascular diseases. With an aging population and socioeconomic and lifestyle changes, the incidence of noncommunicable diseases will continue to increase. NCD-related deaths are projected to increase to 52 million by 2030. In order to reduce the burden of non-communicable diseases, effective measures for the prevention and treatment of non-communicable diseases must be adopted. One of the main causes of non-communicable diseases, including diabetes, hypertension, and atherosclerosis, is the excessive production of reactive oxygen species (ROS) in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/14C07K1/34C07K1/36A61K38/08A61P39/06A23L33/18
CPCA23L33/18A23V2002/00A61K38/00A61P39/06C07K7/06A23V2200/30A23V2250/55
Inventor 张姗姗刘可春韩利文张轩铭李晓彬张云
Owner 山东科桥生物技术有限公司
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