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New method for determination of activity of thiobacillus ferrooxidans

A technology of Thiobacillus ferrooxidans and a new method, which is applied in the direction of microbial-based methods, biochemical equipment and methods, and microbial measurement/inspection, which can solve problems such as low efficiency, complicated operation, and long time, and achieve high efficiency , simple operation, high accuracy effect

Pending Publication Date: 2019-01-11
SHANXI UNIV
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Problems solved by technology

[0016] The present invention aims at the problems of complex operation, long time and low efficiency of the existing method for measuring the activity of Thiobacillus ferrooxidans, and provides a new method for measuring the activity of Thiobacillus ferrooxidans—chemiluminescence method

Method used

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  • New method for determination of activity of thiobacillus ferrooxidans
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  • New method for determination of activity of thiobacillus ferrooxidans

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Embodiment

[0035] The steps of the novel method of measuring Thiobacillus ferrooxidans activity of the present invention are as follows:

[0036] 1. Adjust the parameters of the luminometer to ensure the best signal-to-noise ratio: high voltage 1000V, standard light source 14 The luminous value of C per second is 1500 count / s (luminous intensity / second), the background value is 6 count / s, and the measurement temperature is 30°C.

[0037] 2. Prepare 5 sample cups, dry them after washing, and dry them with lens tissue before use.

[0038] 3. Add 1 mL of luminol analysis solution, H 2 o 2 Solution 0.5mL, draw Fe with a syringe 2+ Standard solution 0.5mL, at the 20s after the start of the measurement, inject Fe from the injection hole at the top of the darkroom 2+ The standard solution is injected into the sample chamber, the total volume of the reaction system is 2mL, the reaction temperature is 30°C, the total reaction time is 100s, and the reaction interval is 0.1s. Add different con...

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Abstract

The invention provides a new method for determination of the activity of Thiobacillus ferrooxidans, and belongs to the field of microbial activity determination, being able to solve the problems of complex method, long time and lower efficiency for determining the activity of Thiobacillus ferrooxidans. The new method includes the steps: using a Chemiluminescence measurement instrument for preparation of Fe2 + standard solution, adding a luminol analytical solution and a H2O2 solution to start timing, injecting Fe2+ standard solutioninto the sample chamber at the 20th second; recording the timetaken for different Fe2+ standard solutions to reach the maximum luminescence value and the cumulative luminescence numb of different Fe2+ standard solutions to reach the maximum luminescence value,and drawing the standard curves of different Fe2+ concentration and luminescence value; inoculating Thiobacillus ferrooxidans into 9K liquid culture medium to determine the content of Fe2+ in the bacterial liquid to be tested; and recording the time taken to reach the maximum luminescence value and the cumulative luminescence number when the maximum luminescence value is reached, and substitutingthe activity of Thiobacillus ferrooxidans in the bacterial liquid to be tested into the standard curve to obtain the activity of the bacterial liquid to be tested. The invention has the advantages ofsimple operation, good sensitivity and high accuracy.

Description

technical field [0001] The invention belongs to the technical field of microbial activity determination, and in particular relates to a new method for determining the activity of Thiobacillus ferrooxidans. Background technique [0002] Acidithiobacillus ferrooxidans (A. ferrooxidans for short) belongs to the kingdom of prokaryotes, the phylum of chemotrophic prokaryotes, the class of bacteria, the family of sulfur bacteria, the genus of Thiobacillus, gram-negative bacteria, and is an obligate chemoautotroph Bacteria, camp aerobic respiration, acidophilus. Widely distributed in soil, sea water, fresh water, sludge, garbage, coal gangue, acid mine water, especially in mine water with huge content. Since Thiobacillus ferrooxidans uses elemental sulfur and ferrous ions as energy substances for growth and metabolism, it is the best desulfurization microorganism for most metal sulfide mine environments or other solid wastes with high sulfur content. And it can produce oxidation,...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12R1/01
CPCC12Q1/06G01N2333/195
Inventor 耿红卓思棋张辰宋立博杨中航董川
Owner SHANXI UNIV
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