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Microfluidic radiation damage biological dosimetry detection device and its detection and analysis method based on luminescent bacteria

A technology of radiation damage and luminescent bacteria, applied in the field of nuclear radiation and space radiation damage biological dose assessment, ultraviolet and ionizing radiation, it can solve the problems of long sample preparation and detection time, lack of blank contrast, poor detection accuracy, etc., to ensure accurate The effect of reducing the cost of detection and use

Active Publication Date: 2020-12-01
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The present invention aims to solve the technical problems of poor detection accuracy caused by long sample preparation and detection time, complex operation, many factors affecting detection, lack of blank comparison, etc. in existing radiation damage biological dose analysis detection equipment and methods, and provides a microscopic method based on luminescent bacteria. Fluidic radiation damage biological dosimetry detection device and its detection and analysis method

Method used

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  • Microfluidic radiation damage biological dosimetry detection device and its detection and analysis method based on luminescent bacteria
  • Microfluidic radiation damage biological dosimetry detection device and its detection and analysis method based on luminescent bacteria
  • Microfluidic radiation damage biological dosimetry detection device and its detection and analysis method based on luminescent bacteria

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specific Embodiment approach 1

[0071] Specific implementation mode one: combine Figure 1~5 This embodiment is specifically described. The microfluidic radiation damage biological dose analysis and detection device based on luminescent bacteria in this embodiment consists of a microfluidic chip 1, an irradiation fixture 2, a condenser lens 3, an optical fiber 4, a photomultiplier tube 5, Composed of pulse counter 6 and device main body 7;

[0072] The microfluidic chip 1 is composed of a chip bottom layer 11, a microchannel layer 12 and a self-adhesive film layer 13, and the chip bottom layer 11, the microchannel layer 12 and the self-adhesive film layer 13 are stacked sequentially from bottom to top; A sample inlet 121, a microchannel 120, a control zone microchamber 122, an irradiation zone microchamber 124, a control zone microchamber outlet 123 and an irradiation zone microchamber outlet 125 are provided, and the sample inlet 121 passes through the microchannel 120 respectively. It is connected with th...

specific Embodiment approach 2

[0076] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the microchamber 122 in the control area and the microchamber 124 in the irradiation area are both circular chambers or consist of a plurality of small chambers or channels. Composed in series. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0077] Specific implementation mode three: combination Figure 6 This embodiment is described in detail. The difference between this embodiment and one of the specific embodiments 1 or 2 is: when the microchamber 122 in the control area and the microchamber 124 in the irradiation area are composed of a plurality of small chambers connected in series , the plurality of small chambers are horizontally arranged side by side and connected in series end to end, the radius of curvature of the microchannel 120 connected between the small chambers is 1mm, and the gap between the small chambers in the longitudinal direction is 1mm. The chambers are all arrow-shaped quadrilaterals, the width of the small chamber is 2mm at the widest, the length of the short side is 1.8mm, the length of the small chamber is 13mm, and the depth is 100 μm; when the microchamber 122 in the control area and the irradiation area When the microchambers 124 are all circular chambers, the diameter of the circula...

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Abstract

The invention discloses a micro-fluidic radiation damage biological dose analysis and detection device based on luminous bacteria and a detection and analysis method thereof and relates to the fieldsof ultraviolet, ionizing radiation, nuclear radiation and space radiation damage biological dose evaluation. The problems existing in the conventional radiation damage biological dose analysis and detection equipment and method that the sample preparation and detection time is long, the operation is complicated, many factors influencing the detection exist and the detection accuracy is low due toabsence of blank reference are solved. The device is composed of a micro-fluidic chip, a radiation fixture, a condensing lens, an optical fiber, a photomultiplier, a pulse counter and a device main body. The method comprises the following steps: loading samples, radiating, loading a chip, detecting and calculating. The device can be composed of a micro-fluidic chip, a radiation fixture, a condensing lens, an optical fiber, an electric optical shutter, a photomultiplier, a pulse counter and a device main body. The method comprises the following steps: loading samples, loading the chip, startingdetection, and recording an initial value, a first radiation and detection cycle, repeating radiation, detecting the cycle and calculating.

Description

technical field [0001] The invention relates to the field of biological dose assessment of ultraviolet radiation, ionizing radiation, nuclear radiation and space radiation damage. Background technique [0002] Radiation, whether it is low-altitude ultraviolet rays, high-altitude space cosmic rays, or even radioactive substances, will unknowingly cause radiation damage to living things. Long-term radiation can cause mental weakness, cancer, lens opacity, and cataracts. [0003] The current radiation damage biological dosimetry methods and instruments have the following problems in the process of use: [0004] First, both the cytoplasmic division arrest micronucleus assay and the conventional culture micronucleus assay require complex operations, long-term culture (more than 70 hours), and a large number of micronucleus observations and statistics under a microscope; [0005] Second, although the detection of serum lipid peroxide and antioxidant activity is relatively simple...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 孙凯李哲煜
Owner HARBIN INST OF TECH
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