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A method for detection of biotin by regulated carbon dots catalyzed SERS by affinity reaction

A biotin and reaction technology, applied in the field of analytical chemistry, can solve the problems of expensive instruments and complicated operations, and achieve the effects of simple method, good selectivity and high sensitivity

Inactive Publication Date: 2020-12-04
GUANGXI NORMAL UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of these reported methods use expensive and cumbersome instruments
To the best of our knowledge, carbon-dot-catalyzed NaH 2 PO 2 -Rh6G reaction produces SERS effect and is used to detect biotin SERS method has not been reported yet

Method used

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  • A method for detection of biotin by regulated carbon dots catalyzed SERS by affinity reaction

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Embodiment

[0023] A method for regulating carbon-dot catalyzed SERS to detect biotin with an affinity reaction, comprising the steps of:

[0024] (1) Prepare VH standard solution system with known concentration: Add 5 μL, 50 μL, 70 μL, 90 μL, 120 μL 0.1ng / mL VH standard solution, 220 μL 0.02 μg / mL Ad solution, 120μL 40μg / L carbon dot solution, 120μL pH 1.5 NaAC-HCl buffer solution, 100μL 0.5mol / L NaH 2 PO 2 solution, 100 μL 10 μmol / L Rh6G solution, 150 μL 0.4 mmol / L AgNR solution and 30 μL 1mol / L NaCl solution, dilute to 1.5 mL with double distilled water, mix well, react in 80 ° C water bath for 20 min, and cool to room temperature in ice water;

[0025] (2) Preparation of blank control solution system: adopt the method of step (1) without adding VH standard solution to prepare blank control solution system;

[0026] (3) Take the VH standard solution system and the blank control solution system prepared according to steps (1) and (2) respectively and pour them into a quartz cuvette. I...

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Abstract

The invention discloses a method for detecting biotin by SERS with affinity reaction-regulated carbon point catalysis. The method comprises the following steps: (1) preparing a biotin standard solution system of known concentration, and measuring a peak intensity value of the SERS at 1617cm-1 as I; (2) preparing a blank control solution system, and also determining the peak intensity value of theSERS as I0; (3) calculating the formula of deltaI=I0-I; (4) using the concentration of deltaI for biotin as a working curve; (5) preparing a to-be-tested sample solution, determining the peak intensity value of the SERS as Isample, and calculating the formula of delatIsample=I0-Isample; and (6) calculating the concentration of biotin in the sample to be tested according to the working curve. The method is simple, rapid, non-toxic in reagent, good in selectivity, and high in sensitivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for detecting biotin by using affinity reaction to regulate carbon point catalyzed SERS. Background technique [0002] Carbon dots refer to new fluorescent carbon nanomaterials with a particle size of less than 10 nm. Because the main elements are carbon, hydrogen, oxygen and nitrogen, and no heavy metal leakage occurs, it is expected to become an ideal substitute for heavy metal semiconductor quantum dots. Due to the advantages of high fluorescence activity, diverse types, good biocompatibility, and low toxicity, carbon dots have been widely used in the fields of biodetection, gene transfer, drug delivery, and bioimaging. The excellent fluorescence properties of carbon dots have shown important application potential in the field of analytical chemistry. At present, there are many methods for preparing carbon dots, which can be roughly divided into two categories: che...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李重宁李丹王丽冰蒋治良梁爱惠
Owner GUANGXI NORMAL UNIV