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A separation and purification method for high-purity phycocyanin

A phycocyanin, separation and purification technology, which is applied in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of complex operation steps, long operation cycle, insufficient purity, etc. Small, high-purity effects

Inactive Publication Date: 2019-01-15
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a method for separation and purification of high-purity phycocyanin, which solves the problems of complicated operation steps, long operation cycle and insufficient purity in the prior art, and has the advantages of good purification effect, cycle time Advantages of short and high yield

Method used

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  • A separation and purification method for high-purity phycocyanin

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for separating and purifying high-purity phycocyanin, comprising the steps of:

[0032] Step 1. Mix the spirulina with the extractant and grind it for at least 30 minutes, then ultrasonically crush it, put it into a centrifuge and centrifuge to obtain the first extract and precipitate, and then use it;

[0033] Step 2, after mixing the precipitation in step 1 with the extractant, carry out ultrasonic crushing, add to the centrifuge and centrifuge to obtain the second extract, mix the second extract with the first extract to obtain the extract, and set aside;

[0034] Step 3, add the ammonium sulfate solution that massfraction is 40% to carry out high-speed centrifugation in described extraction liquid, get supernatant after high-speed centrifugation, then add ammonium sulfate in the supernatant so that the massfraction of ammonium sulfate in the solution is 55% For the above, take the precipitate after medium-speed centrifugation, and set aside;

[0035] Step ...

Embodiment 2

[0046] A method for separating and purifying high-purity phycocyanin, comprising the steps of:

[0047]Step 1. Mix the spirulina with the extractant and grind it for at least 30 minutes, then ultrasonically crush it, put it into a centrifuge and centrifuge to obtain the first extract and precipitate, and then use it;

[0048] Step 2, after mixing the precipitation in step 1 with the extractant, carry out ultrasonic crushing, add to the centrifuge and centrifuge to obtain the second extract, mix the second extract with the first extract to obtain the extract, and set aside;

[0049] Step 3, add the ammonium sulfate solution that massfraction is 40% to carry out high-speed centrifugation in described extraction liquid, get supernatant after high-speed centrifugation, then add ammonium sulfate in the supernatant so that the massfraction of ammonium sulfate in the solution is 55% For the above, take the precipitate after medium-speed centrifugation, and set aside;

[0050] Step 4...

Embodiment 3

[0061] A method for separating and purifying high-purity phycocyanin, comprising the steps of:

[0062] Step 1. Mix the spirulina with the extractant and grind it for at least 30 minutes, then ultrasonically crush it, put it into a centrifuge and centrifuge to obtain the first extract and precipitate, and then use it;

[0063] Step 2, after mixing the precipitation in step 1 with the extractant, carry out ultrasonic crushing, add to the centrifuge and centrifuge to obtain the second extract, mix the second extract with the first extract to obtain the extract, and set aside;

[0064] Step 3, add the ammonium sulfate solution that massfraction is 40% to carry out high-speed centrifugation in described extraction liquid, get supernatant after high-speed centrifugation, then add ammonium sulfate in the supernatant so that the massfraction of ammonium sulfate in the solution is 55% For the above, take the precipitate after medium-speed centrifugation, and set aside;

[0065] Step ...

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Abstract

The invention belongs to the technical field of phycocyanin purification methods, and particularly relates to a separation and purification method for high-purity phycocyanin. The method includes mixing and grinding spirulina and an extractant; performing ultrasonic crushing and centrifugation to obtain a primary extract liquid and precipitate; mixing the precipitate with the extractant and performing ultrasonic crushing and centrifugation to obtain a secondary extract liquid; mixing the primary extract liquid and the secondary extract liquid to obtain an extract liquid; adding an ammonium sulfate solution into the extract liquid; performing high-speed centrifugation; then adding ammonium sulfate into a liquid supernatant to allow the mass percentage of the ammonium sulfate in the solutionto be 55% or above; performing medium-speed centrifugation to obtain precipitates; adding a dissolving agent to dissolve the precipitates; and then performing high-speed centrifugation, millipore filtration, separation of a system with two aqueous phases and purification with an ion exchange column. The method overcomes problems that operation steps are complex, operation periods are long and purity is not high enough in the prior art, and has advantages of good purification effects, short periods and high yields.

Description

technical field [0001] The invention belongs to the technical field of purification methods of phycocyanin, and in particular relates to a separation and purification method of high-purity phycocyanin. Background technique [0002] Phycocyanin is a dark blue powder isolated from spirulina. Mainly found in cyanobacteria, red algae and cryptophyta. Phycocyanin is usually also divided into C-phycocyanin and R-phycocyanin, the former mainly exists in cyanobacteria, the latter mainly exists in red algae, both of which are found in cryptophycobacteria. Its function is to absorb light (orange yellow) energy and transmit light energy. It is not only a kind of protein, but also an excellent natural food coloring, and it is also a good health food. [0003] Phycocyanin is one of the rare pigment proteins in nature. It is not only bright in color, but also a nutritious protein. Its amino acid composition is complete and its content of essential amino acids is high. [0004] Phycocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/36C07K1/34C07K1/30C07K1/18
CPCC07K14/795
Inventor 朱万龙王政昆张浩
Owner YUNNAN NORMAL UNIV