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A method for preparing silica/shellac composite particles containing methylene blue with high fluorescence intensity assisted by heat treatment

A technology of methylene blue and silicon dioxide, applied in chemical instruments and methods, luminescent materials, etc., to achieve the effects of high fluorescence intensity, low toxicity, and easy industrial production

Inactive Publication Date: 2021-04-06
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, the method of preparing silica / shellac composite particles containing methylene blue with high fluorescence intensity by heat-treating silica particles containing methylene blue has not been reported yet.

Method used

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  • A method for preparing silica/shellac composite particles containing methylene blue with high fluorescence intensity assisted by heat treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0016] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

Embodiment 2

[0019] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0020] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

Embodiment 3

[0023] (1) Add 92 ml of absolute ethanol, 17.2 ml of water, 2.48 ml of concentrated ammonia water and 0.10 g of methylene blue to the beaker in sequence. After stirring for 15 minutes, add 3.44 ml of ethyl orthosilicate. After stirring for 4 hours, centrifuge to obtain the sediment , the precipitate was first washed twice with 120 ml of ethanol, and then washed once with 20 ml of acetate buffer solution of pH = 3.6, and then the sediment obtained by centrifugation was dried at 70°C for 24 hours to obtain the Silica particles of methylene blue;

[0024] (2) Dissolving shellac in ethanol to prepare a shellac solution, wherein the concentration of shellac is 10 grams per liter, put the silica particles containing methylene blue prepared in step (1) into 10 ml of shellac In the solution, the concentration of silica particles containing methylene blue was 10 grams per liter, and after ultrasonic treatment for 5 minutes, 30 milliliters of phosphate buffer (pH=7.2~7.4) was added at a...

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Abstract

The invention provides a method for assisting the preparation of methylene blue-containing silica / shellac composite particles with high fluorescence intensity through heat treatment, and belongs to the technical field of chemical raw material preparation. After adding ammonia water, methylene blue and orthosilicate to the mixed solution containing water and ethanol, stir for 4 hours, centrifuge, wash and dry at 70 ° C, and the prepared silica particles containing methylene blue are dispersed in shellac Then, add phosphate buffer solution (PH=7.2~7.4) drop by drop, stir for 1 hour, centrifuge, wash and dry at room temperature, and prepare the silica / shellac composite particles containing methylene blue at 120~ Heat treatment at 180° C. for 24 hours yielded methylene blue-containing silica particles / shellac composite particles with high fluorescence intensity. The method has the advantages of simple process, low raw material price and easy industrial production, and the obtained particles have good biocompatibility and high fluorescence intensity.

Description

technical field [0001] The invention discloses a method for assisting the preparation of methylene blue-containing silica / shellac composite particles with high fluorescence intensity through heat treatment, which belongs to the technical field of chemical raw material preparation. Background technique [0002] Dye molecules have high application value in fluorescence sensors, bioluminescence labeling, cell tracking and solid-state lasers, etc. The realization of these properties is related to the aggregation state of dye molecules. In high-concentration solutions or solid powders, dye molecules, such as methylene blue molecules, are easy to form aggregates, and the interaction between aggregated molecules will lead to the occurrence of fluorescence quenching effect, which will weaken the fluorescence signal, and then cause the fluorescence signal of methylene blue to be almost Unable to detect. Only in dilute aqueous solutions, such as concentrations of methylene blue molec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/06
CPCC09K11/025C09K11/06C09K2211/1037
Inventor 李成峰张伟王文浩王杰葛筱璐
Owner SHANDONG UNIV OF TECH
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