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Biocontrol antagonistic actinomycete for root disease of rubber tree and application thereof
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A technology of actinomycetes and rubber, applied in the field of microbial biocontrol strains, to achieve huge control effect, good bacteriostatic effect, and obvious antagonistic inhibitory effect.
Active Publication Date: 2019-01-15
YUNNAN INST OF TROPICAL CROPS
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Problems solved by technology
Although the existing antagonistic bacteria have certain effects in suppressing rubber tree root disease, they still need to be further improved. How to find new and more effective biocontrol strains has become a technical problem that this field wishes to solve
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Embodiment 1
[0020] (1) Materials
[0021] 1.1 Sample source:
[0022] Rhizosphere soil samples were collected from rubber-planting areas such as Honghe, Dehong, Lincang, and Pu'er in Yunnan Province. When excavating soil, first shovel off the surface soil, dig 100g of soil at a depth of 5-20cm, put it into a ziplock bag, write a label, seal the mouth of the bag, and bring it back to the laboratory.
[0023] 1.2 Medium:
[0024] Soil separation medium is Gao's No. I medium; confrontation experiment medium is potato agarose medium (PDA); activity detection medium YMG (contains malt extract 10.0g in every liter, yeastpowder 4.0g, glucose 4.0g , KH 2 PO 4 2.0g, acid hydrolyzed casein 1.0g, pH 7.2-7.4) and liquid Gowell medium;
[0044] 3.1 Isolation and purification of actinomycetes
[0045] Soil samples were collected from Pu'er (Jiangcheng, Ximeng), Honghe (Hekou, Jinping), Dehong (Ruili, Yingjiang), Lincang (Mengding), the main rubber tree root disease occurrence areas in Yunnan Province, and 44 soil samples were collected 161 strains of actinomycetes were isolated. The aerial hyphae of these strains have different colors such as white, off-white, yellow, orange, orange red, purple black, light green, yellowish brown, blue, black, etc., except for a small number of strains that produce soluble pigments of yellow, yellowish brown, orange red, etc. Also, most strains do not produce.
[0046] 3.2 Preliminary screening of antagonistic actinomycetes
[0047] The plate confrontation method was used for preliminary screening, and an actinomycete strain with good inhibitory effect on two rubber tree root diseases (rubber root fungus and brown root fungus) was screened, ...
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Abstract
The invention provides a high-efficient biocontrol antagonistic actinomycete against rubber tree rootdisease and application thereof, belonging to the microbial field. The biocontrol antagonistic actinomycetes for rubber tree rootdisease provided by the invention belong to Streptomyces mulberry, and are classified as Streptomyces samsunensis 17-7, deposited in China Typical Culture Collection, CCTCC NO: M2018619. As that biocontrol agent prepare by the actinomycete is applied to the root disease of rub tree, It can antagonize many pathogens (white root of rubber, brown root of rubber, etc.),especially against brown root of rubber tree, its inhibition rate is 89.28%, significantly superior to the known antagonistic strains. This biocontrol antagonistic actinomycete has great biocontrol potential in the control of root disease of rubber tree.
Description
technical field [0001] The invention belongs to the technical field of microbial biocontrol strains, and in particular relates to an antagonistic actinomycete strain with high efficiency for biocontrol of rubber tree rootdisease and its application. Background technique [0002] Rubber tree root disease is a kind of disease that seriously harms rubber trees, and the incidence rate of rubber plantations in my country is more than 2%. Rubber tree root disease is a chronic disease, which is sporadic and spread slowly at the beginning of the onset, but the accumulated economic loss is not small after the onset. Root disease causes root rot to the rubber tree, and the absorption function of the lightly diseased plant decreases, which affects the rubber production. When the disease is severe, it can cause the root of the whole tree to rot and eventually lead to the death of the plant. At present, more than 90 kinds of diseases have been found on rubber trees in my country, and r...
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