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An antagonistic actinomycete for biocontrol of rubber tree root disease and its application
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A technology of actinomycetes and rubber, applied in the field of microbial biocontrol strains, to achieve the effect of obvious antagonistic inhibition, huge preventive effect and good antibacterial effect
Active Publication Date: 2021-09-24
YUNNAN INST OF TROPICAL CROPS
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Problems solved by technology
Although the existing antagonistic bacteria have certain effects in suppressing rubber tree root disease, they still need to be further improved. How to find new and more effective biocontrol strains has become a technical problem that this field wishes to solve
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Embodiment 1
[0020] (1) Materials
[0021] 1.1 Sample source:
[0022] Rhizosphere soil samples were collected from rubber-planting areas such as Honghe, Dehong, Lincang, and Pu'er in Yunnan Province. When excavating soil, first shovel off the surface soil, dig 100g of soil at a depth of 5-20cm, put it into a ziplock bag, write a label, seal the mouth of the bag, and bring it back to the laboratory.
[0023] 1.2 Medium:
[0024] Soil separation medium is Gao's No. I medium; confrontation experiment medium is potato agarose medium (PDA); activity detection medium YMG (contains malt extract 10.0g in every liter, yeastpowder 4.0g, glucose 4.0g , KH 2 PO 4 2.0g, acid hydrolyzed casein 1.0g, pH 7.2-7.4) and liquid Gowell medium;
[0044] 3.1 Isolation and purification of actinomycetes
[0045] Soil samples were collected from Pu'er (Jiangcheng, Ximeng), Honghe (Hekou, Jinping), Dehong (Ruili, Yingjiang), Lincang (Mengding), the main rubber tree root disease occurrence areas in Yunnan Province, and 44 soil samples were collected 161 strains of actinomycetes were isolated. The aerial hyphae of these strains have different colors such as white, off-white, yellow, orange, orange red, purple black, light green, yellowish brown, blue, black, etc., except for a small number of strains that produce soluble pigments of yellow, yellowish brown, orange red, etc. Also, most strains do not produce.
[0046] 3.2 Preliminary screening of antagonistic actinomycetes
[0047] The plate confrontation method was used for preliminary screening, and an actinomycete strain with good inhibitory effect on two rubber tree root diseases (rubber root fungus and brown root fungus) was screened, ...
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Abstract
The invention provides a rubber tree rootdisease biocontrol antagonistic actinomycete and application thereof, belonging to the field of microorganisms. The high-efficiency rubber tree rootdisease biocontrol antagonistic actinomycete provided by the present invention belongs to deep-sea Streptomyces, and its classification name is Streptomyces samsunensis 17-7, which is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2018619. The actinomycete is prepared into a biocontrol agent and applied to rubber tree rootdisease, which can have a good antagonistic effect on various pathogenic bacteria (rubber root fungus, brown root fungus, etc.), especially the antagonistic effect on rubber tree brown root fungus. The bacteriostasis rate is 89.28%, which is significantly better than the existing known antagonistic strains, and the biocontrol antagonistic actinomycete has great biocontrol potential in the prevention and treatment of rubber tree root disease.
Description
technical field [0001] The invention belongs to the technical field of microbial biocontrol strains, and in particular relates to an antagonistic actinomycete strain for rubber tree root disease biocontrol and application thereof. Background technique [0002] Rubber tree root disease is a kind of disease that seriously harms rubber trees, and the incidence rate of rubber plantations in my country is more than 2%. Rubber tree root disease is a chronic disease, which is sporadic and spread slowly at the beginning of the onset, but the accumulated economic loss is not small after the onset. Root disease causes root rot to the rubber tree, and the absorption function of the lightly diseased plant decreases, which affects the rubber production. When the disease is severe, it can cause the root of the whole tree to rot and eventually lead to the death of the plant. At present, more than 90 kinds of diseases have been found on rubber trees in my country, and root disease is one o...
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