A method for planting Elymus cylindrica under rain-fed conditions in a typical grassland area
A typical grassland and Elymus cylindrica technology, applied in botany equipment and methods, horticultural methods, chemical instruments and methods, etc., can solve problems such as the survival rate of seedlings that cannot emerge, slow growth of Elymus cylindrica, poor drought resistance of seedlings, etc. , to achieve the effect of increasing the overwintering rate, shortening the cultivation time, and improving the survival rate
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[0060] According to a preferred embodiment of the present invention, step 1 includes the following sub-steps:
[0061] Step 1-1, selecting the seeds of Elymus cylindrica to prepare explants;
[0062] Step 1-2, inoculating the explants obtained in step 1-1 on the induction medium, inducing callus tissue, and subculturing the callus tissue;
[0063] In step 1-3, the callus subcultured in step 1-2 is placed on a differentiation medium and cultivated until it differentiates into clustered shoots;
[0064] In step 1-4, the clustered seedlings obtained in steps 1-3 are placed on the rooting medium and cultivated until young roots grow;
[0065] Step 1-5, transplanting the seedlings that grow radicles in the steps 1-4 to the cultivation soil matrix and continue to cultivate until they grow into finished seedlings;
[0066] In steps 1-6, the finished seedlings in steps 1-5 are hardened to prepare for planting on the grassland.
[0067] According to a preferred embodiment of the pre...
Embodiment 1
[0176] In this embodiment, the cultivation of the Elymus cylindrica seedlings was carried out in an artificial climate chamber.
[0177] Breeding steps:
[0178] (1) Cultivate the seedlings of Elymus cylindrica:
[0179] 1) Select the mature seeds of Elymus cylindrica growing in the typical grassland area of Qilian Mountains, use 1% mercuric chloride to sterilize the surface of the seeds, then wash with sterile double distilled water for 3 times, and select the embryos of the seeds as explants body.
[0180] 2) Inoculate the above-mentioned explants on the induction medium, and the medium formula is: MS, 2,4-dichlorophenoxyacetic acid with a mass concentration of 5 mg / L, 2 mg / L of abscisic acid, 0.05 mg / L The kinetin of L, the sucrose of 25g / L and the agar of 5g / L;
[0181] The calli with a diameter of 2 to 3 cm that are light yellow in color and compact in structure are selected to be peeled off, and inoculated on the subculture medium,
[0182] The formulation of the s...
experiment example 1
[0223] The Elymus cylindrica is planted in embodiment 1 and comparative example 1 when the rainy season ends, statistically analyzes the seedling survival rate of different methods; Measure the overwintering rate of Elymus cylindrica in next spring; area of production. in,
[0224] Survival rate=(number of surviving plants / number of planted plants)×100%;
[0225] Overwintering rate=(plants surviving the winter / number of planted plants)×100%.
[0226] The results are shown in Table 1:
[0227] Table 1 Seedling survival rate, overwintering rate and hay yield per unit area of Elymus cylindrica
[0228]
[0229] As can be seen from the table, in the planting method of Elymus cylindrica described in Example 1, its seedling survival rate has improved by 35.1% compared with Comparative Example 1, and the overwintering rate has improved by 22.5% compared with Comparative Example 1, and the hay yield per unit area is relatively high. Scale 1 improves by 55.6%.
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