Preparation method of mesenchymal stem cell nano-essence liquid

A technology of mesenchymal stem cells and essence, applied in the field of preparation of mesenchymal stem cell nano essence, to achieve the effects of no immune rejection, preventing cell aging, and solving skin aging

Inactive Publication Date: 2019-01-18
溯源生命科技股份有限公司
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, people's research on the causes of human skin aging

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example: Step 1. Cut the tissues on the decidual surface of the placenta, pick the brightly colored tissues, put the tissues into a 10cm dish, cut into meat paste, and spread the cut tissues on T75cm with a cell scraper 2 Slowly add 6mL serum-free medium to the cell culture flask, and put the cell culture flask into a 37℃ incubator with 5% CO 2 , Stand culture; the serum-free medium is Human mesenchymal stem cell basal medium and supplements, the product numbers are: 10151A, 10151B; the two are mixed at 10:1; after mixing, add L-gln to make the final concentration reach 2mM;

[0020] Step 2. After culturing for seven days, change half of the medium, and then change half of the medium. When the cell confluence reaches about 80%, perform subculture and freeze the P2-P6 generation cells;

[0021] Step 3. Resuscitate P3, P4, P5, P6 generation cells in mesenchymal stem cell culture medium at 37℃ with 5% CO 2 Culture in an incubator, when the cells grow to 80% confluence, collect l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of mesenchymal stem cell nano-essence, in particular to the cosmetic chemical field which utilizes mesenchymal stem cell and nano-technology to prepare anti-aging essence. The preparation method comprises the following steps: cutting the decidua surface tissue of placenta fundus, culturing for seven days, half-changing medium, resuscitating P3, P4, P5,P6 passage cells in mesenchymal stem cell culture medium, ultrasonic disintegrating; Adding essence, mixing the culture medium of P3, P4, P5, P6 passage cells to obtain B phase; C phase is obtain by mixing a mixture of glycerol, sorbitol, hydrolyze oat protein, water and glycerol with water; C phase is heat to 80 DEG C and uniformly stirred. A phase and B phase are added, that mixture is uniformlystirred and then the material is filtered out. Placental mesenchymal stem cells (PMSCs) are widely used and suitable for large-scale production. Nano-technology is used to form ultramicro-capsule. And the active ingredient of PMSCs is encapsulated in the core of micro-capsule, which can make the active ingredient more lasting and solve the problem of skin aging from the root.

Description

technical field [0001] The invention relates to a method for preparing a mesenchymal stem cell nano-essence, in particular to the field of cosmetic chemistry that uses mesenchymal stem cells and nanotechnology to prepare and form an anti-aging essence. Background technique [0002] With the improvement of living standards and the advancement of science and technology, people are urged to pursue a more perfect self, and the aging population has also made anti-aging a topic of concern to more people. According to Kline, a world-renowned consulting and research company, anti-aging products continue to maintain strong growth momentum, with more than half of the market focusing on anti-aging products that can delay or reduce the effects of aging. In addition, Euromonitor International's research report pointed out that anti-aging is currently the most popular trend in the Asian market, and they predict that anti-aging products will still be the focus of attention in the Asian mar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K8/99A61K8/98A61K8/64A61K8/34A61K8/11A61Q19/00A61Q19/08C12N5/0775
CPCA61K8/11A61K8/345A61K8/645A61K8/982A61K8/99A61K2800/56A61Q19/00A61Q19/08C12N5/0662C12N5/0668
Inventor 汪晓敏袁卫平万谦
Owner 溯源生命科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap