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Method for extracting chrysin-8-C-Beta-D-glucoside from Camellia semiserrata Chi

A technology of glucoside and chrysin, applied in the field of extracting chrysin-8-C-β-D-glucoside, which can solve the problems of insufficient research and achieve the effect of simple extraction method and high extraction purity

Active Publication Date: 2019-01-18
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, not enough research has been done on the specific components in the extract

Method used

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  • Method for extracting chrysin-8-C-Beta-D-glucoside from Camellia semiserrata Chi
  • Method for extracting chrysin-8-C-Beta-D-glucoside from Camellia semiserrata Chi
  • Method for extracting chrysin-8-C-Beta-D-glucoside from Camellia semiserrata Chi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic extraction with 50% ethanol aqueous solution at 60°C for 3 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), and then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 20 times with water to obtain a sample liquid.

[0047] Shelling and degreasing are to maximize the extraction yield and reduce interference during the separation process. Appropriate raw material handling methods are also beneficial to avoid the loss of target compounds.

[0048] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 110L), rinse with ethanol and water in turn until there is no e...

Embodiment 2

[0054] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching with 60% ethanol aqueous solution at 70°C twice, each time for 2 hours, with a solid-liquid ratio of 1:2 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 22 times with water to obtain a sample liquid.

[0055] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of BV 1 The volume fractio...

Embodiment 3

[0060] The tea fruit of Nanshan tea is dried in the air, shelled, and the cake produced after removing oil by physical pressing is dried in the air, and properly crushed to obtain Nanshan tea cake. Ultrasonic leaching with 55% ethanol aqueous solution at 65°C for 4 times, 2 hours each time, with a solid-liquid ratio of 1:4 (m / v), then filtered and combined to obtain the extract. Then the extract was concentrated, extracted with an equal volume of n-butanol to obtain an extract, and then diluted 18 times with water to obtain a sample liquid.

[0061] Choose the model of macroporous resin to be D101 type, first soak D101 macroporous resin with ethanol, then pack column (column volume BV 1 10L), rinse with ethanol and water in turn until there is no ethanol smell, and it can be detected with an alcohol meter, and the reading is less than 5. Slowly add the sample solution into the macroporous resin chromatography column, and then sequentially use 6 times of BV 1 The volume fract...

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Abstract

The invention provides a method for extracting chrysin-8-C-Beta-D-glucoside from Camellia semiserrata Chi. The method comprises the following steps: a Camellia semiserrata Chi cake is extracted, the sample solution is loaded onto a macroporous resin chromatographic column, and after gradient elution, eluent eluted by 15 percent aqueous ethanol solution is collected and post-processed, so that a crude component solution is obtained; the crude component solution is sampled onto a polyamide resin chromatographic column, and after gradient elution, eluent containing the target compound is collected, concentrated and freeze-dried, so that a crude extract is obtained; a high performance liquid chromatograph and a C18HCE chromatographic column are used for carrying out DAC (Dynamic Axial Compression) preparation, so that a crude separated product is obtained; the high performance liquid chromatograph, a C18ME chromatographic column and aqueous acetonitrile solution (the volume fraction is 19percent) as a mobile phase are used for carrying out isocratic elution, the flowing part containing the target compound is collected, and by concentration and freeze-drying, the target compound is obtained. The method provided by the application effectively separates out the target compound from the Camellia plant for the first time, and the purity of extraction is high.

Description

technical field [0001] The invention relates to the field of compound extraction, in particular to a method for extracting chrysin-8-C-β-D-glucoside from Nanshan tea. Background technique [0002] Nanshan tea, also known as Guangning safflower oil tea (Camellia semiserrata Chi.), belongs to the plant of the genus Camellia (Camellia L.) of the family Theaceae (Theaceae), and is a perennial tree or shrub. Nanshan tea is mainly distributed in the southeast of Guangxi and the west of Guangdong in China. It is an important cultivated species of camellia oleifera. After research and comparison, it is found that among all kinds of Camellia oleifera, the chemical components in Guangning safflower Camellia oleifera are relatively rich. [0003] Nanshan tea and its cakes are especially rich in compounds such as flavonoids and saponins with various biological activities. At present, the research on Camellia oleifera cake mainly focuses on the optimization of various extraction proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07
CPCC07H1/08C07H17/07
Inventor 王元清袁虎严建业张晓帆曹清明
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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