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Device and method for cultivating photosynthetic microorganisms using sunlight

A technology of photosynthetic microorganisms and sunlight, applied in the direction of microorganism-based methods, enzymology/microbiology devices, biochemical cleaning devices, etc., can solve the problems of light transmission, mass transfer, transmission, cleaning and microbial pollution, and cannot be cultivated efficiently Photosynthetic microorganisms, cumbersome operation, etc., to avoid uneven aeration, improve microalgae cultivation efficiency, and simple device structure

Active Publication Date: 2021-08-06
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Consuming a large amount of conventional nitrogen sources is expensive for cultivating microalgae. If a useful nitrogen source can be obtained from a large amount of industrial NOx emissions, the problem of nitrogen fertilizer sources for large-scale algae cultivation can be solved, but this requires the use of algae cultivation. Nitrogen source consumption rate matches exhaust gas denitrification rate
[0016] To sum up, the existing technologies cannot comprehensively solve the problems of light transmission, mass transfer, transmission, cleaning and microbial contamination, or consume a lot of energy and cost, or are too complicated and cumbersome to operate, so they cannot be large-scale and high-efficiency. photosynthetic microorganisms

Method used

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  • Device and method for cultivating photosynthetic microorganisms using sunlight
  • Device and method for cultivating photosynthetic microorganisms using sunlight
  • Device and method for cultivating photosynthetic microorganisms using sunlight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] This embodiment is used to illustrate the effect of the device and method of the present invention.

[0142] use image 3 The apparatus shown is for culturing spirulina. During the cultivation process, φ is 0°, θ is 80°; I max About 60000lux ~ 160000lux.

[0143] Use Zarrouk culture medium, and sterilize at 120°C for 30 minutes, cool down and set aside. Enter the culture medium into each culture space, turn on the air compressor, and let in air at a ventilation rate of 0.7L / L·min (the gas volume is based on the standard state). The static pressure at the bottom of the closed breeding space is about 7.5KPa, and the aeration hole starts to aerate when the pressure in the aeration pipe is 9.75KPa. Insert spirulina for cultivation. When the pH value of the algae solution is greater than 10.5, CO 2 , when the pH value of the algae solution is less than 8.5, stop feeding CO 2 . The culture temperature is controlled between 26°C and 35°C by spraying water on the surfac...

Embodiment 2

[0152] This embodiment is used to illustrate the effect of the device and method of the present invention.

[0153] Adopt the method and condition identical with embodiment 1, cultivate identical spirulina in the same day, the difference with embodiment 1 is only: adopt Figure 4 device shown.

[0154] At the end of the cultivation, the OD values ​​of the eight closed culture spaces were 8.95, 9.35, 9.29, 9.18, 9.83, 9.25, 9.80, and 9.04 respectively; the growth curve of Spirulina in a closed culture space in the middle was as follows: Figure 7 shown.

Embodiment 3

[0162] This embodiment is used to illustrate the effect of the device and method of the present invention.

[0163] Using the same device, method and conditions as in Example 2, the same spirulina was cultivated on the same date, the difference is only: start the circulation pump, drive the culture solution to circulate between eight closed culture spaces, and the circulation of the culture solution It is 1.5L / min.

[0164] At the end of the cultivation, the OD value of the eight closed culture spaces was 10.65, and the growth curve of Spirulina in one of the closed culture spaces was as follows: Figure 7 shown.

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Abstract

The invention relates to a device for cultivating photosynthetic microorganisms by using sunlight, comprising: (1) a closed culture space composed of flexible transparent film bags; (2) several inlets and outlets for gas and liquid transportation; An aeration device at the bottom of the space; and (4) a bracket, the bracket is formed by two supporting surfaces arranged in a Λ shape; the angle between the supporting surface and the horizontal plane is θ, and θ is greater than 30° to less than 90°, The angle between the intersection line of the support surface and the horizontal plane and the north-south direction is φ, and φ is 0°-15°, and the support surface is used to support the closed breeding space. The invention also relates to a method for cultivating photosynthetic microorganisms and a method for producing biomass and bioenergy using the device. The device and method of the invention are more suitable for large-scale and high-efficiency cultivation of photosynthetic microorganisms.

Description

technical field [0001] The invention relates to a device and a method for cultivating photosynthetic microorganisms by using sunlight, in particular to a device and a method for cultivating photosynthetic microorganisms on a large scale by using sunlight. Background technique [0002] Photosynthetic microorganisms are a class of organisms that can use light for photosynthesis, such as photosynthetic bacteria and microalgae. Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by sunlight, and absorbs CO through efficient photosynthesis of microalgae cells. 2 , convert light energy into chemical energy of carbohydrates such as fat or starch, and release O 2 . The use of microalgae to produce bioenergy and chemicals is expected to simultaneously replace fossil energy and reduce CO 2 emissions etc. Due to the extremely high production efficiency of microalgae, it has received wide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12N1/12C12P1/00B01D53/84B01D53/56C11B1/10C10G1/04C12R1/89
CPCB01D53/56B01D53/84B01D2251/95C10G1/04C10G2300/1014C11B1/10C11B1/104C12M21/02C12M23/14C12M23/34C12M23/48C12M29/06C12M41/12C12M41/26C12N1/12C12P1/00Y02A50/20
Inventor 荣峻峰黄绪耕周旭华朱俊英程琳纪洪波
Owner CHINA PETROLEUM & CHEM CORP